水貂部分Toll样受体(TLRs)基因的分子克隆、序列分析及组织表达分析

doi:10.16431/j.cnki.1671-7236.2015.08.001

Abstract

Abstract: The present study was aimed to explore the potential role of Toll-like receptors (TLRs) on the mink immunity, and accumulate alternative genetic material for the mink breeding for disease-resistant.Four pairs of primers were designed according to ferret TLR sequences in GenBank to obtain the sequences of TLR genes (TLR4, TLR6, TLR7 and TLR8) of mink and then performed bioinformatics analysis of these sequences.In addition, the expression of TLR4 and TLR7 genes in various tissues in young and adult minks were analyzed by RT-PCR. Sequence homology analysis showed that mink had higher homology with carnivora (ferrets, polar bear, panda, walrus, seals, dog, tiger and cat) which had the nearest perimeter in the phylogenetic tree.Tissue expression analysis showed that TLR4 and TLR7 were widely and differently expressed in variety tissues of mink.Interestingly, the expression of TLR4 and TLR7 in young mink were much higher than that in adult mink.

Key words: Toll-like receptors; tissues expression; mink; sequence analysis

CLC Number:

Q786

TONG Ming-wei, YI Li, YANG Yong, CHENG Yue-ning, WANG Jian-ke, ZHAO Hang, LIN Peng, CHENG Shi-peng. Molecular Cloning,Sequence Analysis and Tissues Expression Profile of Part of Mink Toll-like Receptors (TLRs) Genes[J]. , 2015, 42(8): 1917-1924.

References

[1] 曾爽,何小兵,陈国华,等.Toll样受体介导的信号转导通路及其调控分子的研究进展[J].中国兽医科学,2011,41(4):433-440.
[2] Kawai T,Akira S.The role of pattern-recognition receptors in innate immunity:Update on Toll-like receptors[J].Nat Immunol,2010,11(5):373-384.
[3] 马思慧,杨欢,吴天成,等.Toll样受体信号传导通路的研究进展[J].中国畜牧兽医,2014,41(8):160-164.
[4] Iwasaki A,Medzhitov R.Regulation of adaptive immunity by the innate immune system[J]. Science,2010,327(5963):291-295.
[5] Hans M,Hans V M.Toll-like receptors and their dual role in periodontitis:A review[J].J Oral Sci,2011,53(3):263-271.
[6] Kondo T,Kawai T,Akira S.Dissecting negative regulation of Toll-like receptor signaling[J].Trends Immunol,2012,33(9):449-458.
[7] 胥静,丁力,张俊平.Toll样受体和其他分子识别受体在固有免疫中的相互作用[J].药学实践杂志,2014,32(5):324-328.
[8] Basu M,Swain B,Maiti N K.Inductive expression of Toll-like receptor 5 (TLR5) and associated downstream signaling molecules following ligand exposure and bacterial infection in the Indian major carp,mrigal (Cirrhinus mrigala)[J].Fish Shellfish Immunol,2012,32(1):121-131.
[9] Nakamoto M,Moy R H,Xu J,et al.Virus recognition by Toll-7 activates antiviral autophagy in Drosophila[J].Immunity,2012,36(4):658-667.
[10] Alvarez B,Revilla C,Chamorro S,et al.Molecular cloning,characterization and tissue expression of porcine Toll-like receptor 4[J].Dev Comp Immunol,2006,30(4):345-355.
[11] Wang K,Mu Y.Molecular characterization and expression analysis of Toll-like receptor 1 from large yellow croaker (Pseudosciaena crocea)[J].Fish Shellfish Immunol,2013,35(6):2046-2050.
[12] Chang J S,Russell G C,Jann O,et al.Molecular cloning and characterization of Toll-like receptors 1-10 in sheep[J]. Vet Immunol Immunopathol,2009,127(1-2):94-105.
[13] White S N,Taylor K H,Abbey C A,et al.Haplotype variation in bovine Toll-like receptor 4 and computational prediction of a positively selected ligand-binding domain[J]. Proc Natl Acad Sci USA,2003,100(18):10364-10369.
[14] Alemu S W,Bijma P,Mller S H,et al.Indirect genetic effects contribute substantially to heritable variation in aggression-related traits in group-housed mink[J].Genet Sel Evol,2014,46(1):30-35.
[15] Asahina Y,Yoshioka N,Kano R,et al.Full-length cDNA cloning of Toll-like receptor 4 in dogs and cats[J].Vet Immunol Immunopathol,2003,96(3-4):159-167.
[16] Okui Y,Kano R,Maruyama H,et al.Cloning of canine Toll-like receptor 7 gene and its expression in dog tissues[J].Vet Immunol Immunopathol,2008,121(1-2):156-160.
[17] Muzio M,Bosisio D,Polentarutti N,et al.Differential expressionand regulation of Toll-like receptors (TLR) in human leukocytes:Selective expression of TLR3 in dendritic cells[J]. J Immunol,2000,164(11):5998-6004.
[18] Visintin A,Mazzoni A,Oberg H H,et al.Regulation of Toll-like receptors in humanmonocytes and dendritic cells[J].J Immunol,2001,166(1):249-255.
[19] Broonstra A,Assalin-Paturel C,Gilliet M,et al.Flexibility of mouse classical and plasmacytoid- derived dendritic cells in directing Thelper type 1 and 2 celldevelopment:Dependency on antigen dose and differential Toll-like receptor ligation[J]. J Exp Med,2003,197(1):101-109.
[20] 陈煜,时红波,张帆,等.TLR2、TLR4和TLR9在慢性重型肝炎患者及肝衰竭大鼠中的表达[J].世界华人消化杂志,2010,18(13):1320-1325.

Molecular Cloning,Sequence Analysis and Tissues Expression Profile of Part of Mink Toll-like Receptors (TLRs) Genes

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[2]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[3]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[4]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[5]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[6]	GUO Jinxiang, LYU Huiru, LI Jiaofeng, LIAO Chengshui, ZHANG Linlin, NIU Junhui. Antibacterial Activity of Licochalcone A Against Pseudomonas aeruginosa Isolated from Mink [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1181-1188.
[7]	YU Xiaojun, LI Dandan, LI Xuewu, WANG Qun, WANG Guang, WANG Lihua. Effects of Antibacterial Peptide on the Immune Function and Intestinal Tissue Morphology of Growing Female Minks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 443-453.
[8]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[9]	CHEN Qiang, CHENG Yuening, FENG Qiuju, GUO Li, ZHANG Shuqin, TAN Bin, YI Li, ZHAO Quan, CHENG Shipeng, SUN Na. Isolation, Identification and Drug Resistance Analysis of Klebsiella pneumoniae from Minks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 700-708.
[10]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[11]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[12]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[13]	ZHANG Qingxun, JING Shengfan, HAN Shuyi, WANG Ruoqi, FENG Shengyong, HE Hongxuan. Isolation,Identification,Drug Resistance and Virulence Genes Detection of Pseudomonas aeruginosa from Mink [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2230-2237.
[14]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[15]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.