牛奶中雌二醇胶体金试纸条快速检测技术研究

doi:10.16431/j.cnki.1671-7236.2017.11.034

Abstract

Abstract:

In order to develop a sensitive and specific method for the detection of estradiol residues (E₂) in milk, a colloidal gold immunochromatographic method was established. Colloidal gold particles were prepared by trisodium citrate reduction method and labeled with rabbit polyclonal antibodies (PcAb) by physical adsorption method. After optimization of reaction conditions such as the amount of coating antigen and labeled antibody to assemble test strip, the sensitivity, specificity, repeatability and accelerated preservation of the test strip were determined. The estrogen analogue cross reaction and milk matrix interference reaction were also measured. The results showed that the optimized concentration of E₂-OVA antigen was 2 mg/mL and the optimized antibody concentration was 20 μg/mL, visual detection limits of E₂ was 10 μg/L in PBS within 10 min. The cross reaction rate of this method with estriol was 40%, there were no negligible cross-reactivities with other estrogen compounds including estradiol valerate, estradiol benzoate, estrone, diethylstilbestrol, quinestrol, ethinyloestradiol and nonylphenol. Milk samples only needed two times diluted before analysis, and the results could judge by naked eye after 10 min with cut-off of 20 μg/L. The results demonstrated that the developed method was suitable for rapid on-site screening of E₂ residues in milk samples.

Key words: estradiol (E₂); colloidal gold immunochromatography assay; rapid detection; milk

CLC Number:

S859.84

BAI Yu, ZHANG Jing, HU Jing-yan, XU Yong-peng, WANG Shu-ming. Study on Colloidal Gold Immunochromatographic Assay for Rapid Detection of Estradiol in Milk[J]. , 2017, 44(11): 3351-3357.

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Study on Colloidal Gold Immunochromatographic Assay for Rapid Detection of Estradiol in Milk

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