移植骨髓间充质干细胞对萨能奶山羊乳腺组织发育及血清激素水平的影响

doi:10.16431/j.cnki.1671-7236.2017.01.010

Abstract

Abstract:

The study was aimed to analyze the effect of transplanted bone marrow mesenchymal stem cells (BMSCs) on the mammary gland development and serum hormone level of Saanen dairy goat.16 healthy goats with 2 months old and similar body weight were randomly divided into control group and experimental group. The BMSCs which was amplified to the P6 generation was injected to the goat via jugular vein,and the control group was injected with the same amount of normal saline. Feeding and management of the two groups were the same. Blood samples were collected monthly,and mammary gland tissue samples were collected at 3,5 and 7 months old. Frozen sections of mammary gland tissue was prepared, the ducts and acinus numbers were observed and recorded. The results showed that after transplantation of BMSCs,at 5 and 7 months old,the ducts and acinus number of goat in experimental group were significantly higher than that of control group (P<0.05).At 5,7,8 and 9 months old,the serum E₂ level of goat in experimental group was significantly higher than that of control group (P<0.05);At 5 to 9 months old,the serum P level in experimental group was significantly higher than that of control group (P<0.05).At 7 to 9 months old,the serum PRL and INS levels in experimental group were significantly higher than that of the control group (P<0.05);At 8 and 9 months old,the serum levels of GnRH level in experimental group was significantly higher than that of the control group (P<0.05).The serum LEP,CRH and ACTH levels were higher than that of the control group at the whole test process,but the difference was not significant (P>0.05).In conclusion, injection of BMSCs could increase the duct and acinus number of dairy goat mammary,improve the hormone level in serum,and promote mammary gland development.

Key words: BMSCs; duct; acinus; hormone; mammary gland development

CLC Number:

S826.9⁺4

SHAO Wei, LI Yang, WANG Li-wen, LI Cui, Muheyizha·Rehenbieke, YU Xiong. Effect of Bone Marrow Mesenchymal Stem Cells Transplantation on the Mammary Tissue Development and Serum Hormone Level of Saanen Dairy Goat[J]. , 2017, 44(1): 74-79.

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Effect of Bone Marrow Mesenchymal Stem Cells Transplantation on the Mammary Tissue Development and Serum Hormone Level of Saanen Dairy Goat

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