›› 2016, Vol. 43 ›› Issue (7): 1890-1897.doi: 10.16431/j.cnki.1671-7236.2016.07.035

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Study on the Antidiarrhea Effect of Four Kinds of Tibetan Veterinary Drug Prescription

HE Dong-mei, HU Qiao, TIAN Yu-shi, WEI Yu-qing, XU Jing-wei, CHEN Chao-xi   

  1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • Received:2015-12-11 Online:2016-07-20 Published:2016-07-22

Abstract:

The experiment was done in order to understand the effect of four kinds of Tibetan veterinary drug extracts on the antibacterial diarrhea,and to guide the clinical treatment of the disease.Using K-B disk diffusion method and broth dilution method,the antibacterial effect of drug extracts of Veronica ciliate Fisch,Usneadiffracta Vain.,Sophoraflavescens var.flavescens and Lamiophlomisrotata (Benth.) Kudo on fourteen kinds of common pathogenic bacteria were detected.Four kinds of Tibetan veterinary drug prescription were optimized by orthogonal design software and the mice model was established by clinical isolates of Salmonella blegdam.According to the LD50of four kinds of Tibetan veterinary drug optimization prescription,it was divided into three groups of 0.060,0.030 and 0.020 g/mL,respectively.The results showed that the four kinds of Tibetan veterinary drug on most trials of pathogenic bacteria had good inhibitory effect.The Usneadiffracta Vain had good inhibitory effect on all the test bacteria.Four Tibetan veterinary drug optimization prescription of high dose on mice with bacterial diarrhea showed the best therapeutic effects,there was conducive to the treatment of the clinical disease.

Key words: Tibetan veterinary drug; extract; antibacterial effect; prescription; LD50; anti diarrhea; bacterial diarrhea model

CLC Number: