鸭致病性大肠杆菌分离鉴定及其相关致病性分析

doi:10.16431/j.cnki.1671-7236.2016.01.034

Abstract

Abstract: To analyze the pathogenicity and biological characteristics of duck E.coli, 27 strains of duck pathogenic E.coli were isolated from dead ducks with the typical characterize of colibacillosis by isolation and culture, physical and chemical properties identification in Xichang city.O serotype identification, drug sensitivity test and virulence associated genes were detected in 27 strains of duck pathogenic E.coli.The result showed that the predominant serotypes were O119, O86, O126, O142 and O44, which accounted for 55.56%.O119 was the epidemic and pathogenic serotype in this farm, which accounted for 40.74% in 27 strains of duck pathogenic E.coli.Through the drug sensitivity test of 20 kinds of clinical commonly used drugs, we found that all strains were sensitive to amikacin, gentamicin and polymyxin B.Besides, 10 drugs(ceftriaxone, etc) were lightly sensitive.However, these strains were resistant to 5 kinds of drugs(rifampicin, etc).The detection result of PCR about virulence associated genes indicated that the positive of iutA, hlyF, Iss, IroN, ompT, fyuA, irp 2, Tsh and papA genes were 100.00%, fimC and K 99 genes were 59.26% and 7.40%, respectively.The results provided an important reference for effective prevention and control of duck colibacillosis, and laid the foundation for further study of E.coli.

Key words: duck pathogenicE.coli; isolation and identification; drug resistance; pathogenicity

CLC Number:

S852.61⁺4

DENG Bo-xiong, LIU Qing, DENG An-yuan, LI Wen-wen, SONG Zhi-gang, YU Qiong, CHEN Guang-fen, LIU Shu-huang, LIU Ya-gang. Isolation, Identification and Related Pathogenicity Analysis of Duck Pathogenic E.coli[J]. , 2016, 43(1): 235-241.

References

[1] 陈伯伦.鸭病[M].北京:中国农业出版社,2008.
[2] Zanella A,Alborali G L,Bardotti M,et al.Severe Escherichia coli O111 septicaemia and polyserositis in hens at the start of lay[J]. Avian Pathology,2000,29(4):311-317.
[3] Ewers C,Li G,Wilking H,et al.Avian pathogenic,uropathogenic,and newborn meningitis-causing Escherichia coli:How closely related are they[J]. International Journal of Medical Microbiology,2007,297(3):163-176.
[4] Cho Y I,Kim W I,Liu S,et al.Development of a panel of multiplex real-time polymerase chain reaction assays for simultaneous detection of major agents causing calf diarrhea in feces[J]. J Vet Diagn Invest,2010,22(4):509-517.
[5] Johnson T J,Wannemuehler Y,Doetkott C,et al.Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool[J]. Journal of Clinical Microbiology,2008,46(12):3987-3996.
[6] 于学辉,程安春,汪铭书,等.鸭源致病性大肠杆菌的血清型鉴定及其相关毒力基因分析[J].畜牧兽医学报,2008,39(1):53-59.
[7] 吴信明,连洪梅,王艳,等.38株鸭源大肠杆菌的分离鉴定与药敏试验[J].中国畜牧兽医,2010,37(10):178-180.
[8] 胡青海,刘晓文,苗晋锋,等.当前我国鸭病的流行动态与防制对策[J].中国禽业导刊,2001,23:18.
[9] 李玲,汪铭书,程安春,等.雏鸭致病性大肠杆菌的分离鉴定药敏试验及耐药性分析[J].中国兽医杂志,2007,43(12):34-36.
[10] 张永信.远东地区细菌耐药状况与对策[J].中国抗生素杂志,1994,5:391-392.
[11] 白灏,冀辉,韩先干,等.禽致病性大肠杆菌江苏、安徽分离株的生物学特性分析[J].微生物学通报,2013,40:1315-1322.
[12] 陆承平.兽医微生物学[M].第5版.北京:中国农业出版社,2013.
[13] 王亚君,樊琛,李一经. fimC基因与鸡源大肠杆菌致病性的相关[J].畜牧兽医学报,2004,35(6):680-684.
[14] 任士飞,徐建生,董国雄,等.细菌黏附研究进展[J].中国预防兽医学报,2004,26(3):238-240.
[15] 张英,董国雄,江美娟.对FC株猪源性肠毒素型大肠杆菌致病因子的研究[J].江苏农学院学报,1990,21(1):67-73.
[16] Smets B F,Barkay T.Horizontal gene transfer:Perspectives at a crossroads of scientific disciplines[J]. Nature Reviews Microbiology,2005,3:675-678.

Isolation, Identification and Related Pathogenicity Analysis of Duck Pathogenic E.coli

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