冷刺激小鼠脂肪组织差异表达长链非编码RNA的初步研究

doi:10.16431/j.cnki.1671-7236.2015.11.008

Abstract

Abstract: Adipose tissue had two different types:White adipose tissue (WAT) that stored energy and brown adipose tissue (BAT) which could consume energy by specific expression of uncoupling protein 1(UCP1).In contrast with BAT,WAT had strong plasticity and possessed capacity of conversing phenotype,which was called thermogenesis (browning) under cold exposure.According to the results of RNA transcriptome sequencing of interscapular brown adipose tissue (iBAT) and subcutaneous white adipose tissue (sWAT) in the cold exposure group and control group,we preliminary screened one differentially expressed lncRNA (lncRNA-6030408B16Rik).The lncRNA-6030408B16Rik expression pattern was detected by qRT-PCR in spleen,muscle,kidney,duodenum,brain,liver,sWAT and iBAT from the wild type mice.Twenty-four 8-week-old C57BL/6 wild type mice were randomly divided into two groups respectively under cold exposure 6℃ and 28℃ as control,followed by qRT-PCR detection of lncRNA-6030408B16Rik expression in sWAT and iBAT.Separating preadipocytes from 1-day-old wild type mice,then inducing differentiation,and collecting cells on 0,1,3 and 5 days,expression of UCP1 and lncRNA-6030408B16Rik were detected by qRT-PCR.The results showed that the expression of lncRNA-6030408B16Rik were greatly enriched both in sWAT and iBAT compared with other six tissues.The expression of lncRNA-6030408B16Rik were significantly or extremely significantly up-regulated both in sWAT and iBAT of mice under cold exposure compared to control group (P<0.05;P<0.01).In contrast with UCP1,lncRNA-6030408B16Rik expression level presented an up-down trend at 0,1,3 and 5 days of brown preadipocytes differentiation.lncRNA-6030408B16Rik showed tissue-specific expression both in sWAT and iBAT,these results suggested that it might play important roles in WAT "browning" and brown preadipocytes differentiation.

Key words: mice; white adipose tissue; brown adipose tissue; preadipocytes differentiation; long non-coding RNA (lncRNA)

CLC Number:

HUANG Su-juan, TAO Cong, ZHEN Er-dong, WANG Ya-jun, WEI Gang, BAI Li-jing, YANG Shu-lin, WANG Yan-fang, LI Kui, AO Hong. Preliminary Study on Differentially Expressed Long Non-coding RNA in Cold-exposure Adipose Tissue of Mice[J]. , 2015, 42(11): 2867-2872.

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Preliminary Study on Differentially Expressed Long Non-coding RNA in Cold-exposure Adipose Tissue of Mice

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