Abstract: Quantitative detection of the clenbuterol in pig urine was investigated with a clenbuterol kit based on immunofluorescence chromatography and a fluorescence quantitative detector,and the sensitivity,accuracy,precision,specificity,stability of the strip were evaluated.The results showed that the quantitative curve fitting was performed by using four parameters method,the linear coefficient R² was 0.99972.The detecting limit was up to 0.1 μg/L.The recovery rate of 3 different concentration levels were 80% to 120%.The intra assay coefficient of variation were 12.2%,11.1% and 15.2%,respectively.The cross reaction with the 4 β-agonists and 8 antibiotics was less than 0.01%.The experiment confirmed that the kit had 1 year shelf-life.The total detection process just needed 15 min.

Key words: clenbuterol; fluorescent quantitative immunochromatographic method; detection