巢式PCR检测H1亚型禽流感病毒方法的建立

Abstract

Abstract: In order to develop a simple and quick method to detect H1 subtype avian influenza virus (AIV),two pairs of primers were designed according to the sequences of HA gene of H1 subtype AIV, and a nested polymerase chain reaction (nPCR) method was developed for detection of H1 subtype AIV. The specificity and sensitivity test showed that this nPCR was only specific to the H1 subtype AIV and could detect 13.2 fg/μL of AIV RNA. The results demonstrated that the optimized nPCR assay was rapid, specific and sensitive for detection of H1 subtype AIV.

Key words: H1 subtype avian influenza virus; nested PCR; detection

CLC Number:

GUO Jie, XIE Zhi-xun, LUO Si-si, LIU Jia-bo, DENG Xian-wen, XIE Zhi-qin, PANG Yao-shan, FAN Qing. Establishment of a Nested PCR Technique for Detection of H1 Subtype Avian Influenza Virus[J]. , 2013, 40(9): 23-26.

References

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Establishment of a Nested PCR Technique for Detection of H1 Subtype Avian Influenza Virus

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