小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱的分子生物学鉴定

Abstract

Abstract: This study is to establish a method for identifying Hyalomma anatolicum(H.anatolicum), Hyalomma asiaticum(H.asiaticum)and Hyalomma detritum(H.detritum)with molecular markers and to learn the phylogenetic relationship of these ticks. Ticks were collected from domestic animals in Xinjiang and Inner Mongolia provinces and classified by morphology characters. Then the 16S rRNA and COⅠ of ticks were amplified by PCR and subsequently sequenced. Phylogenetic trees were constructed by Mega 5.0 and Mrbayes 3.2. On the phylogenetic trees based on the 16S rRNA, H.anatolicum and H.aisaticum were clustered together with their respective classes. And H.detritum was clustered with their respective class and the H.anatolicum, H.asiaticum formed distinct branches on the phylogenetic trees based on the COⅠ. The method based on morphology that combined with molecular 16S rRNA and COⅠ seemed a simple, accuracy method for the identification of H.anatolicum, H.asiaticum and H.detritum

Key words: cytochrome oxidaseⅠ; 16S rRNA; Hyalomma anatolicum; Hyalomma asiaticum; Hyalomma detritum; molecular identification

CLC Number:

LÜ Ji-zhou, WU Shao-qiang, ZHANG Yong-ning, WANG Zhen-bao, FENG Chun-yan, WANG Cai-xia, DENG Jun-hua, YUAN Xiang-fen, LIN Xiang-mei. Molecular Identification of Hyalomma anatolicum, Hyalomma asiaticum and Hyalomma detritum[J]. , 2013, 40(6): 7-14.

References

1 邓国藩. 中国经济昆虫志[M]. 第15册. 北京:科学出版社,1978.
2 邓国藩. 中国经济昆虫志[M]. 第39册. 北京:科学出版社,1991.
3 王振宝, 吾仁其米克, 巴音查汗,等. 小亚璃眼蜱的鉴定及其生物学特性研究[J]. 新疆农业大学学报,2009, 32(2): 63~66.
4 孙明,曾巧英,殷宏,等. 实验室条件下小亚璃眼蜱生活史观察[J]. 中国媒介生物学及控制杂志,2011,22(4):344~347.
5 陈泽,杨晓军,杨晓红,等. 中国蜱类地理分布及区系分析[J]. 四川动物,2008,27(5):820~823.
6 杨晓军,陈泽,刘敬泽. 蜱类系统学研究进展[J]. 昆虫学报,2007,50(9):941~949.
7 杨晓军,陈泽,刘敬泽. 中国蜱类有效属和有效种[J]. 河北师范大学学报(自然科学版),2008,32(4):529~533.
8 罗建勋,殷宏,关贵全,等. 残缘璃眼蜱培育及环形泰勒虫的实验性传播[J]. 中国兽医科技,2002,32(6):27~28.
9 罗建勋,殷宏,关贵全,等. 小亚璃眼蜱和亚洲璃眼蜱对环形泰勒虫传播能力的研究[J]. 中国兽医科技,2003,33(2):22~23.
10 阿力米江·沙克,巴音查汗. 阿合奇县色乡牦牛环形泰勒虫病流行病学调查报告[J]. 当代畜牧, 2012, 4:25~27.
11 张思华. 蜱与蜱传疾病最新研究进展[J]. 安徽预防医学杂志,2012,1(4):45~48.
12 康发亮. 蜱分类的方法及我国蜱分类的研究进展[J]. 安徽农业科学,2011,39(33):20495~20497,20500.
13 徐广,方庆权. 分子系统进化关系分析的一种新方法——贝叶斯法在硬蜱属中的应用[J]. 动物学报,2003,49(3):380~388.
14 Anderson J M, Ammerman N C, Norris D E. Molecular differentiation of metastriate tick immatures. Vector Borne Zoonotic Dis,2004, 4: 334~342.
15 Apanaskevich D A, Horak I G. The genus Hyalomma. Ⅺ. Redescription of all parasitic stages of H. (Euhyalomma) asiaticum (Acari: Ixodidae) and notes on its biology. Exp Appl Acarol,2010, 52(2):207~220.
16 Caporale D A, Rich S M, Spielman A, et al. Discriminating between ixodes ticks by means of mitochondrial DNA sequences. Mol Phylogenet Evol, 1995, 4: 361~365.
17 Chen Z, Yu Z, Yang X, et al. The life cycle of Hyalomma asiaticum kozlovi Olenev, 1931 (Acari: Ixodidae) under laboratory conditions. Vet Parasitol,2009, 160(1~2):134~137.
18 Chu K H, Li C P, Qi J. Ribosomal RNA as molecular barcodes:A simple correction analysis without sequence alignmen. Bioinformatics,2006,22(14):1690~1701.
19 Klompen J S H, Black IV W C, Keirans J E, et al. Systematics and biogeography of hard ticks, a total evidence approach. Cladistics, 2000, 16: 79~102.
20 Mangold A J, Bargues M D, Mas-Coma S. Mitochondrial 16S rDNA sequences and phylogenetic relationships of species of Rhipicephalus and other tick genera among Metastriata. (Acari: Ixodidae). Parasitol Res,1998, 84: 478~484.
21 Mixson T R, Lydy S L, Dasch G A, et al. Inferring the population structure and demographic history of the tick, Amblyomma Americanum Linnaeus. J Vector Ecol, 2006, 31: 181~192.
22 Nabian S, Rahbari S, Changizi A, et al. The distribution of Hyalomma spp. ticks from domestic ruminants in Iran. Med Vet Entomol,2009, 23(3):281~283.
23 Rees D J, Dioli M, Kirkendall L R. Molecules and morphology: Evidence for cryptic hybridization in African Hyalomma (Acari: Ixodidae). Mol Phylogenet Evol,2003, 27: 131~142.
24 Ronquist F, Huelsenbeck J P. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 2003, 19:1572~1574.
25 Schwan T G, Hinnebusch B J. Bloodstream- versus tick-associated variants of a relapsing fever bacterium. Science, 1998, 280: 1938~1940.
26 Shemshad K, Rafinejad J, Kamali K,et al. Species diversity and geographic distribution of hard ticks (Acari: Ixodoidea: Ixodidae) infesting domestic ruminants, in Qazvin province, Iran.Prasitol Res, 2012,110(1):373~380.
27 Tamura K, Peterson D, Peterson N, et al. Mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011,28: 2731~2739.

[1]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[2]	JIANG Changjin, CHEN Yun, PAN Pengcheng, CHEN Baojian, GUAN Zhihui, LU Huilin, XIE Bingkun, QIN Zhaoxian. Fecal Microflora Structure and Its Correlation with Growth Rate in Finishing Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 924-931.
[3]	QIAO Hongxing, DING Yong, ZHANG Liheng, SONG Yuzhen, DONG Qing, BIAN Chuanzhou, ZHAO Junqiang. Isolation, Identification, 16S rRNA Gene Sequencing and Genetic Evolution Analysis of Aeromonas aeruginosa from Duck [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 435-442.
[4]	ZHANG Yuying, LIU Shujie, FENG Yuzhe, ZHANG Xiaowei, CUI Zhanhong. Bacterial Flora Composition of Normal and Diarrhea Yak Calves Based on 16S rRNA Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3293-3302.
[5]	SONG Shengnan, GUO Jia, YANG Yicheng, SANG Chunli, YANG Meihua, WANG Yuanzhi. Species Identification of Ticks from Pet Dog and Detection of Brucella Carried by Ticks in Shihezi Area [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1717-1724.
[6]	ZHONG Rui, HE Tingmei, BI Bo, YANG Xingtao, SHI Xiaogang, JIN Senlong, FENG Gang, PENG Guangneng. Isolation,Identification and Drug Resistance Analysis of Enterococcus faecalis from Raccoon [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1112-1120.
[7]	GUO Wenjie, LIU Shujie, CUI Zhanhong, SUI Lu. Comparative Analysis of Gut Bacterial Composition of Grazing Male Yaks at Different Ages Based on 16S rRNA Sequencing Technology [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3614-3624.
[8]	SI Jinglei, HUANG Ye, CHEN Kuirong, LYU Dongling, CHENG Feng, TIAN Weilong, LIU Xiaoxiao, LIANG Liang, LI Yueyue, FENG Lingli, GAO Jiuyu, MO Jiayuan, LIANG Jing, LAN Ganqiu. Evaluating of the Association Between Early-stage Weight and Gut Microbiota in Duroc Pigs Using 16S rRNA Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 190-199.
[9]	ZHANG Zijing, ZHU Xiaoting, LYU Shijie, JIN Lei, XU Jiawei, HUANG Yongzhen, LI Zhiming, WANG Xianwei, YU Xiang, YANG Shang, LI Jing, WANG Eryao, XU Zhaoxue, SHI Qiaoting. Study on the Structure and Function of Fecal Microbiome Between Diarrhea and Healthy Calves [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2779-2788.
[10]	YAN Chaohua, WEN Ming, ZHOU Bijun, ZHANG Xu, CHEN Guoquan, ZHAO Dajie, ZHANG Tiantian, WANG Na, LIU Yanhan. Isolation,Identification and Drug Resistance Analysis of Aerococcus viridans from Rhizomys sinensis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2643-2651.
[11]	TANG Haibo, YUN Chenxia, LIANG Liang, YUAN Xiaofang, HUANG Zhengtuan, CAO Yinyin, LENG Jing. Isolation and Identification and Biological Characterization of Aeromonas hydrophila from Tupaia belangeri yaoshanensis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1560-1570.
[12]	WU Qiong, WANG Sizhen, ZHANG Shi, HU Zongfu, YANG Hao, NIU Huaxin. Analysis of Rumen Microbial Diversity and Functional Prediction of Chinese Simmental Cattle Based on 16S rRNA High-throughput Sequencing Technology [J]. , 2019, 46(5): 1370-1378.
[13]	LING Chen, HAO Chengwu, HE Hai, ZHANG Fei, HOU Feng, HE Sun. Isolation and Identification of Mycoplasma bovis in Xinjiang [J]. , 2019, 46(5): 1466-1473.
[14]	FANG Zhenhua, DAI Xiaomei, DING Li, HONG Meiling. Isolation,Identification and Drug Susceptibility Analysis of Pseudomonas putida from Chinemys reevesii [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3115-3123.
[15]	ZHANG Mingyang, HU Andong, CHENG Zhentao, JIANG Haibo, WEN Ming. Isolation,Identification and Drug Resistance Analysis of Plesiomonas shigelloides from Hybrid Surgeon [J]. , 2019, 46(1): 264-270.

Molecular Identification of Hyalomma anatolicum, Hyalomma asiaticum and Hyalomma detritum

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments