牛支原体间接免疫酶标组织化学检测方法的建立

Abstract

Abstract: To establish indirect immuno-histochemical method for detection of Mycoplasma bovis(M.bovis), the pure culture of M.bovis and the lung from the calve infected with the M.bovis were immuno-histochemically studied by using the chicken anti M.bovis IgY and goat anti-chicken IgG-HRP as the first and second antibody, and optimizing the staining conditions to determine the best immuno-histochemistry staining method. The method was used to detect the diseased sections of dead calves under optimal conditions, and the result was verified by isolation and PCR methods. The immuno-histochemistry positive specimens in line with isolation and PCR positive results, while the negative control and alternative test were negative. It showed that the indirect immuno-histochemical method established in this trial could be used to specifically and reliably detect M.bovis of tissue samples and culture in the clinical cases,it also could be provided to explore the site in the organism, the regularity of dynamic distribution and the mechanism of pathogenicity of this pathogen.

Key words: immuno-histochemistry; detection; M.bovis

CLC Number:

S858.23

JIN Yun-yun, YAN Gen-qiang, WANG Jing-mei, SHI Gang. Establishment of the Indirect Immuno-histochemical Method for Detecting Mycoplasma bovis[J]. , 2013, (5): 26-30.

References

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Establishment of the Indirect Immuno-histochemical Method for Detecting Mycoplasma bovis

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