番鸭呼肠孤病毒套式RT-PCR检测方法的建立及应用

Abstract

Abstract: According to the GenBank login duck reovirus genome sequence, using biology software to design and synthesis two pairs of primers, established a nest RT-PCR detection method for the detection of Muscovy duck reovirus(MDRV), and used the detection method of the isolated virus and other poultry virus for application testing. The results showed that the method could amplify specific target fragment from MDRV, sensitivity to 0.1 pg virus RNA, while other viruses of avian reovirus (ARV), chicken infectious bursal disease virus (IBDV), Muscovy duck parvovirus(MDPV), goose parvovirus (GPV), duck hepatitis virus (DHV) and sample amplification results were negative. Therefore, the research provided a reference for MDRV disease detection and diagnose.

Key words: Muscovy duck reovirus; nest RT-PCR; detection

CLC Number:

S852.65

LI Qi-qiang. Establishment and Application of A Nest RT-PCR Method for Detection of Muscovy Duck Reovirus[J]. , 2012, 39(10): 83-86.

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Establishment and Application of A Nest RT-PCR Method for Detection of Muscovy Duck Reovirus

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