马兜铃酸Ⅰ对大鼠五脏的毒性及功能影响

doi:10.16431/j.cnki.1671-7236.2022.07.034

Abstract

Abstract: 【Objective】 This study was aimed to explore the toxicity and functional effects of aristolochic acid Ⅰ (AA-Ⅰ) on the five viscera (heart,liver,spleen,lung and kidney) of rats,so as to provide reference for the clinical use of traditional Chinese medicines of the Aristolochia genus in the future.【Method】 SD rats were randomly divided into aristolochic acid administration and blank control groups.The rats in administration group were gavaged with AA-Ⅰ at 20 mg/kg for 7 days.At 0.25 h,0.5 h,1 h,2 h,24 h,and 15 d after drug withdrawal,the five viscera of rats were taken.HPLC method was used to detect the contents of AA-Ⅰ and aristolactams-Ⅰ (AL-Ⅰ) in five viscera of rats at each time point,and the main physiological functions and histopathological changes of five viscera of rats were detected.【Result】 At 0.25 h after administration,AA-Ⅰ was mainly distributed in heart,liver,spleen and lung,and AL-Ⅰ was detected in liver and spleen.At 0.5 h after administration,AA-Ⅰ was mainly distributed in the heart,liver,spleen,lung and kidney.Except for the heart,the content of AA-Ⅰ in the other four viscera reached a high peak,and AL-Ⅰ was detected in liver,lung and kidney.At 1 h after administration,AA-Ⅰ was mainly distributed in liver and lung,and AL-Ⅰ was detected in liver.At 2 h after administration,AA-Ⅰ was mainly distributed in heart,liver,lung and kidney,and AL-Ⅰ was detected in kidney.At 24 h after administration,AA-Ⅰ was mainly distributed in heart and liver,and AL-Ⅰ was detected in liver.After 15 d of drug withdrawal,AA-Ⅰ and AL-Ⅰ were not detected in five viscera.The results of function tests showed that AA-Ⅰ could cause oxidative stress injury in five viscera.【Conclusion】 AA-Ⅰ was administered to rats at a dose of 20 mg/kg for 7 d, different levels of AA-Ⅰ were detected in five viscera,and AL-Ⅰ was detected in liver,kidney,lung and spleen.AA-Ⅰ could cause different degrees of damage to five viscera,and its damage was related to oxidative stress.

Key words: aristolochic acid Ⅰ (AA-Ⅰ); aristolactams-Ⅰ(AL-Ⅰ); rats; five viscera; toxicity

CLC Number:

S853.7

WANG Yao, YAN Ge, LIU Zhihui, WANG Yu, XIN Quanchao, CAO Xinyue, YU Wenhui, JIANG Xiaowen. Study on the Toxicity and Function Effects of Aristolochic Acid Ⅰ on the Five Viscera of Rats[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2757-2767.

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Study on the Toxicity and Function Effects of Aristolochic Acid Ⅰ on the Five Viscera of Rats

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