Abstract: The purpose of this experiment was to study the anti-inflammatory and analgesic effects of Pulu Ruyan San,and to provide the basis for the later clinical development.The anti-inflammatory effect was studied by xylene induced ear swelling,acetic acid induced peritoneal capillary permeability and carrageenan induced toe swelling in mice.The analgesic effect was studied by formalin and hot plate apparatus.In each experiment,mice were randomly divided into 5 groups, 10 mice in each group, half male and half female, which were negative control group, low, medium and high dose groups of Pulu Ruyan powder, and aspirin positive control group (aspirin group). The low, medium and high-dose groups of Pulu Ruyan powder and aspirin group were given 20, 30, 40 and 0.15 mg/g crude drugs by gavage once a day, respectively. The negative control group was given the same volume of normal saline by gavage for 7 days, and the follow-up was carried out according to different experiments.The results of mouse auricle swelling test showed that compared with the negative control group,each medication group could extremely significantly reduce the auricle swelling induced by xylene (P<0.01),but compared with aspirin group,there was no significant difference between Pulu Ruyan San middle and high-dose groups (P>0.05).The results of acetic acid induced peritoneal capillary permeability test showed that compared with the negative control group,each medication group had extremely significant inhibitory effect on acetic acid induced permeability increase (P<0.01).Compared with aspirin group,the difference between Pulu Ruyan San low and middle dose groups was very extremely significant (P<0.01),while the difference between high dose group was not significant (P>0.05).The results of carrageenan induced toe swelling test showed that compared with the negative control group,each medication group had different degrees of swelling inhibition (P<0.01).Compared with aspirin group,the difference was extremely significant in Pulu Ruyan San low dose group (P<0.01),but the difference was not significant in the middle and high-dose groups (P>0.05).The results of formalin induced pain test showed that compared with the negative control group,the times of licking feet in each treatment group were extremely significantly reduced in phase Ⅰ (P<0.01).In phase Ⅱ,the differences between each group were significant or extremely significant (P<0.05;P<0.01).The results of hot plate instrument pain test showed that compared with the negative control group,the pain threshold of each medication group was significantly prolonged at each time after administration (P<0.01).Compared with aspirin group,the effect of reducing pain threshold of Pulu Ruyan San high dose group was extremely significantly stronger than that of aspirin group (P<0.01),and the effects of Pulu Ruyan San low and middle dose groups were extremely significantly weaker than that of aspirin group (P<0.01).The results showed that Pulu Ruyan San had good anti-inflammatory and analgesic effects on mice.

Key words: Pulu Ruyan San; decoction and alcohol precipitation; anti-inflammatory; analgesia