Abstract: he objective of this study was to explore the effects of buffalo follicular fluid (BuFF) and bovine follicular fluid (BoFF) on the IVF of buffalo oocytes obtained from Ovum pick-up (OPU) and abattoir，in order to develop IVF system and improve embryo production efficiency in buffalo. According to the replacement amount of fetal bovine serum (FBS) by FF in IVM medium，oocytes were distributed randomly into 4 groups. group Ⅰ: 0% FF+ 10% FBS，group Ⅱ: 5% FF +5% FBS，group Ⅲ: 10% FF+ 0% FBS and group Ⅳ: 15% FF+ 0% FBS. As for addition of BuFF，the results showed that BuFF did not improve the cleavage rate of OPU and abattoir-derived oocytes after IVF (P>0.05). However，addition of 5% and 10% BuFF have significantly improved the develop efficiency of IVF embryos，the blastocyst formation rates of groups Ⅱ and Ⅲ were significantly higher than those of groups Ⅰand Ⅳ(P＜0.01)，and there was no difference between groups Ⅱ and Ⅲ (P＞0.05). The blastocyst formation rate of group Ⅳ was lower than that of group Ⅰ，but the difference between two groups were not significant (P>0.05). As for the addition of BoFF，the cleavage rate and blastocyst formation rate of OPU-derived oocytes in group Ⅲ were significantly higher than those of groups Ⅰand Ⅳ(P＜0.01)，and there was no significant difference between groupsⅠand Ⅱ(P>0.05). BoFF did not improve the cleavage rate of abattoir-derived oocytes after IVF (P＞0.05). However，the blastocyst formation rate of groups Ⅱand Ⅲ were significantly higher than those of groups Ⅰ(P<0.05) and Ⅳ (P＜0.01)，and blastocyst formation rate of group Ⅳ was significantly lower than that of group Ⅰ(P<0.05)，while the blastocyst formation rate was significantly different between groups Ⅱand Ⅲ(P＞0.05).In conclusion，addition of 5% to 10% BuFF or BoFF in IVM medium in place of FBS can significantly improve the efficiency of IVF embryo production in buffalo，and BuFF shows a better effect.

Key words: BuFF; BoFF; buffalo oocytes; IVF