China Animal Husbandry & Veterinary Medicine ›› 2008, Vol. 1 ›› Issue (10): 86-88.

• 疾病防治 • Previous Articles     Next Articles

Study on Detection of Avian Influenza Virus H9 Subtype by Dot-ELISA

JIAO Xianqin1， LIAO Zhonglei2， CHEN Hongying2，3，HAN Yulin4, WANG Lei5，LI Xinsheng2,3
   

  1. 1.Zhengzhou Modern Agricultural Technology Service Centre, Zhengzhou 450002, China;  2.The College of animal Husbandry And Veterinary，Henan Agricultural University， Zhengzhou 450002， China； 3.Animal Food Safety Key Laboratory，Henan Province，Zhengzhou 450002， China； 4.Husbandry and Veterinary Burearu of Jiaozuo，Jiaozuo 454150， China; 5.Husbandry and Veterinary Burearu of Xingyang,Xingyang 450100, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-20 Published:2008-10-20

Abstract: A Dot-ELISA method was developed for the detection of avian influenza virus (AIV) H9 subtype. The optimum working concentration of rabbit anti-AIV IgG was determined to be 1∶400,and that of goat anti-rabbit IgG labeled with horseradish peroxidase(HRP)to be 1∶400 too. The assay could detect as low as 3.35×10-9g /disc. Comparison of the Dot-ELISA with HA and HI, AGP, virus isolation were conducted. In the Dot-ELISA, 36 of 63 were positive (57.14%), 38 of 63 in the virus isolation were positive (60.30%). The high specify of the Dot-ELISA was shown by the specific blocking test with AIV positive serum and cross-reaction test with newcastle disease virus, infectious bronchitis virus, infectious bursal disease virus, egg drop syndrome virus. The result of test reproducibility in the Dot-ELISA was very good (93.9%). The procedure took about 3 h, and it was economical, results of reaction could be easily read by eye. This rapid and inexpensive method could be proved to be a new diagnostic technique for early diagnosis of avian influenza.

Key words: Dot-ELISA; detection; H9 subtype; avian influenza virus

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