Abstract: Antimicrobial resistance test of 19 samonella isolates was done by disc dilution method(NCCLS 2003).According to the construction of I integron , The drugresistance rate of amikacin,kanamycin, gentamicin,neomycin ,streptomycin, ampicillin, doxycycline，norfloxacin, ceftriaxone, chloramphenicol, sulfafurazole, tetracycline, cefazolin，was 5.26%,63.16%,47.37%,36.84%,84.21%,84.2%,100%,57.9%,5.26%,52.6%,89.47%,100%, 26.3% respectively. Most of the isolates were multidrug resistance and the rate was 84.21%(16/19).Among the total of the multidrugresistance isolates,the rate of resistance to five ,six, seven, eight ,nine and ten drugs was 10.53%，5.26%，15.79%，21.05%，15.79%， 15.79%. PCR was employed to amplify I integron and analysis the relationship between I integron and multidrug resistance. 3 pairs of primer was design to amplify I integron's intI1 gene, variable segment and 3'conserved segment. As a result, we amplified several fragments with different length from 750 to 2000 bp. Among these 19 isolates, the detection rate of I integron was 73.68%（14/19）; and among these 16 isolates which had been proven multidrugresistant,the detection rate of I integron was 87.5%.The I integron positive isolates' rate of multidrugresistance was 100%(14/14) . The results shows I integron obviously has the relationship with multidrug resistance . 

Key words: samonella; I integron; multidrug resistance; detection