N-氨基甲酰谷氨酸对绒山羊胎盘发育影响的研究

doi:10.16431/j.cnki.1671-7236.2025.06.021

Abstract

Abstract: 【Objective】 The objective of this study was to investigate the effects of dietary supplementation of N-carbamyl glutamate (NCG) on placental morphology,newborn litter weight and body size of lambs,plasma antioxidant indexes,and vascular endothelial growth factor (VEGF) in blood and placenta，in order to understand the role of NCG in placental development of cashmere goats.【Method】 60 ewe of Liaoning cashmere goat,weighing (45.0±2.3) kg and in good condition,aged 8 months,were selected,and artificially inseminated after estrus at the same time,and the ewes conceived at the same time were recorded by B-ultrasound examination technology.They were randomly divided into control group (CON，total mixed diet) and NCG group (total mixed diet containing 0.09% NCG).The pre-trial period was 1 week and the formal period was 22 weeks.At the end of the 18th week of the experiment,blood samples were collected from the jugular vein of pregnant ewes,and plasma antioxidant indexes were determined by the kit.The placenta was collected after the end of pregnancy,the weight of placenta,the weight and number of villous leaves were calculated,the uniformity and density of villous leaves were calculated,the newborn litter weight and newborn weight of lambs were recorded,and the body height,body length and chest circumference of lambs were measured.【Result】 Compared with CON group,the placenta weight,newborn litter weight and newborn weight and chest circumference of lambs in NCG group were significantly increased (P＜0.05).The contents of nitric oxide (NO),VEGF and vascular endothelial growth factor A (VEGFA) and the activity of glutathione peroxidase (GSH-Px) in plasma of ewe in NCG group were significantly increased (P＜0.05).The contents of VEGF and VEGFA in placenta of ewe in NCG group were significantly lower than those in CON group (P＜0.05).【Conclusion】 NCG could affect the antioxidant capacity of pregnant cashmere goats and increase the level of VEGF through NO pathway,thus promoting the development of placenta and fetus.The results provided a theoretical basis for the application of NCG in the diet of cashmere goat ewes.

Key words: cashmere goats; N-carbamylglutamate (NCG); placenta; vascular endothelial growth factor (VEGF)

CLC Number:

S827.5

LI Mingqian, CHANG Shuai, MA Guixia, HAN Di, QUAN Zhiguo, DOU Xingtang, MA Wei, WANG Chunqiang. Study on the Effect of N-carbamoylglutamate on Placental Development in Cashmere Goats[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2684-2690.

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Study on the Effect of N-carbamoylglutamate on Placental Development in Cashmere Goats

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