饲粮代谢能水平对盐津乌骨鸡雏鸡肠道发育及盲肠微生物的影响

doi:10.16431/j.cnki.1671-7236.2024.11.014

Abstract

Abstract: 【Objective】 The aim of this experiment was to study the effects of dietary metabolizable energy level on the intestinal development and cecal microbial diversity of Yanjin Silky chickens aged 1-42 days. 【Method】 A total of 200 healthy 1-day-old Yanjin Silky chickens with similar body weight were randomly divided into 5 groups with 5 replicates in each group and 8 chickens in each replicate.The chickens were fed five experimental diets with metabolizable energy levels of 11.40,12.00,12.60,13.20 and 13.80 MJ/kg,respectively.At 42 days of age,one chicken was taken from each replicate for slaughter,each intestinal segment was separated and its length and mass were determined,and the contents of the cecum were taken to analyze the diversity of cecal microbial flora by the Illumina NovaSeq high-throughput sequencing platform. 【Result】 The mean cecal length of Yanjin Silky chickens in 11.40 and 12.00 MJ/kg groups were significantly higher than that of 13.20 and 13.80 MJ/kg groups (P＜0.05).The absolute and relative mass of rectum in 11.40 MJ/kg group was significantly higher than that in 12.60 and 13.80 MJ/kg groups (P＜0.05).Dietary metabolizable energy level had no significant effect on cecal microbial Alpha diversity index of Yanjin Silky chickens (P＞0.05),but the microbial community structure in 11.40 MJ/kg group was obvious different from that in 12.60,13.20 and 13.80 MJ/kg groups.At the phylum level,Firmicutes,Bacteroidetes and Desulfurobacteria were the dominant phyla in the caecum of the five groups of Yanjin Silky chickens,and the relative abundance of Cyanobacteria of 12.00 MJ/kg group was significantly higher than that in 11.40, 12.60 and 13.80 MJ/kg groups (P＜0.05).At the genus level,the dominant genera in the caecum of the five groups were Faecalibacterium,Bacteroides,Alistipes and unclassified_Oscillospiraceae,and the relative abundance of [Ruminococcus]_torques_group in 11.40 and 12.60 MJ/kg groups were significantly higher than that in 13.20 and 13.80 MJ/kg groups (P＜0.05). 【Conclusion】 Under these experimental conditions,the dietary metabolizable energy levels of 13.20 and 13.80 MJ/kg could inhibit the development of caecum and rectum and the growth of some pathogenic bacteria to a certain extent.Dietary metabolizable energy level had little effect on cecal microbial Alpha diversity of Yanjin Silky chickens,but it could affect the structure of cecal microbial community and caused changes in the dominant microbial flora and their relative abundance.

Key words: metabolizable energy level; Yanjin Silky chickens; intestinal development; microbial diversity

CLC Number:

S831.5

TANG Anxing, ZHANG Zhengfei, SUN Jindong, FU Lixiang, WANG Xingxian, ZHANG Shiyun, LI Cien, YANG Liangyu, TAO Linli, NIU Guoyi. Effects of Dietary Metabolizable Energy Levels on Intestinal Development and Cecal Microbial of Yanjin Silky Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 4792-4801.

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Effects of Dietary Metabolizable Energy Levels on Intestinal Development and Cecal Microbial of Yanjin Silky Chickens

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