广东省猪十二指肠贾第虫感染现状和分子特征分析

doi:10.16431/j.cnki.1671-7236.2022.04.042

Abstract

Abstract: 【Objective】 This study was aimed to evaluate the zoonotic risk of Giardia duodenalis (G.duodenalis) in pigs,and investgate the prevalence and molecular characterization of G.duodenalis in different citys of Guangdong province.【Method】 Fresh pig feces samples were collected from 10 regions in Guangdong province and examined by microscope.Nested PCR was used to amplify the glutamate dehydrogenase (gdh) gene of G.duodenalis,and the positive rates of G.duodenalis in different regions and growth stages were calculated according to the amplification results.All PCR positive products were sequenced,and the gdh gene sequences of G.duodenalis were BLAST compared to determine the genotype of G.duodenalis.The maximum likelihood method of Mega 7.0 software was used to construct the evolutionary tree.【Result】 The cyst of G.duodenalis was oval with thick wall and the size was (10-14) μm×(7-10) μm.It had an obvious longitudinal axis column,and the nucleus was distributed on both sides of the axis column.94 positive samples were detected from 521 samples,and the average positive rate was 18.04%(94/521),among which Maoming (40.00%,10/25) and Qingyuan (35.06%,27/77) had higher positive rate,but Zhaoqing and Shaoguan were lower,with 3.90%(3/77) and 8.57%(3/35) respectively.The positive rates in Foshan,Jiangmen,Yangjiang,Heyuan,Guangzhou and Huizhou were 11.11%(2/18),15.31%(15/98),11.43%(4/35),13.33%(4/30),9.38%(3/32) and 24.47%(23/94),respectively.The positive rate of G.duodenalis in sows (24.55%) was significantly higher than that in weaned piglets (13.30%)(P<0.05),but there was no significant difference between the positive rate of G.duodenalis in sows and fattening pigs (19.23%)(P>0.05).Based on genotyping and phylogenetic analysis of gdh gene,a total of 5 G.duodenalis subtypes were found,including assemblage AⅠ and assemblage E1,E2,E10 and E13,of which 61.70% (58/94) G.duodenalis positive samples belonged to sub-assemblage AⅠ and 38.30% (36/94) belonged to assemblage E.【Conclusion】 The infection of G.duodenalis from pigs in Guangdong province was common,and the positive rates of pigs in different regions and different growth stages were different.The zoonotic sub-assemblage AⅠ was the dominant sub-assemblage,indicating that there was a risk of transmission from animals to humans or vice versa and the possible public health problems caused by the G.duodenalis should be taken seriously.

Key words: pig; Giardia duodenalis; gdh gene; infection rate; zoonotic risk

CLC Number:

S855.9

ZHONG Menglong, ZHANG Haoji, CHEN Xiangjie, LI Fukun, QIU Xixi, LI Yi, HUANG Fuqiang, WANG Nina, YU Xingang. Infection Status and Molecular Characterization of Giardia duodenalis in Pigs in Guangdong Province[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1592-1598.

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Infection Status and Molecular Characterization of Giardia duodenalis in Pigs in Guangdong Province

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