Abstract: 【Objective】 The traditional Chinese medicine compound consisting of Salvia miltiorrhiza,Leonurus heterophyllus, Scutellaria baicalensis and Forsythia suspensa was analyzed by network pharmacology,in order to study the possible targets of its effective components for treating dairy cow endometritis,and explain the mechanism of target action pathway.【Method】 TCMSP database was used to screen the target of drug components,NCBI,GeneCard,geneMap,TTD and DrugBank were used to find the related targets of cow endometritis,and the common gene targets of drug components and cow endometritis were screened out,which were used as the predicted targets for the treatment of cow endometritis with this traditional Chinese medicine compound,and then uploaded to STRING database to establish PPI network,which was screened by Cytoscape 3.8.2 software according to the connectivity.Metascape database was used to analyze the enrichment of GO function and KEGG pathway of core targets,and its mechanism was explored.【Result】 133 kinds of effective active ingredients were screened out from the traditional Chinese medicine compound,mainly including quercetin,kaempferol,baicalein,tanshinone ⅡA,etc.,292 corresponding target proteins were found through drug ingredients,130 related targets of cow endometritis were found through database,and 24 predicted targets of traditional Chinese medicine compound for treating cow endometritis were screened out,among which 7 core targets were screened out,including tumor necrosis factor,protein kinase and prostaglandin-endoperoxide synthase 2.GO function enrichment analysis resulted in 20 items of biological process,6 items of molecular function and 6 items of cell component.Biological processes included the reaction of lipopolysaccharide,the positive regulation of leukocyte-cell adhesion,the reaction to inorganic substances,etc.Molecular function included signal receptor activity regulation,transcription coactivator binding,heme binding and so on.Cell components included cell membrane raft,membrane side,granular secretory cavity,etc.The enrichment analysis of KEGG pathway involved 20 genes,and there were 9 pathways involved in regulation.Among them,many genes included AGE-RAGE signaling pathway,cancer proteoglycan pathway,blood flow shear stress and atherosclerosis pathway and Th17 cell differentiation pathway.【Conclusion】 The traditional Chinese medicine compound might have therapeutic effect on cow endometritis by regulating tumor necrosis factor,protein kinase,prostaglandin-endoperoxide synthase 2 and other targets,and by participating in AGE-RAGE signaling pathway and Th17 cell differentiation.

Key words: traditional Chinese medicine compound; network pharmacology; dairy cow; endometritis; action mechanism