鹿场环境中细菌的分布及耐药性调查

doi:10.16431/j.cnki.1671-7236.2017.03.039

Abstract

Abstract:

The experiment was aimed to identify the bacterial distribution and drug resistance situation in deer farm environment form Xifeng county, Liaoning province. We collected manure, water, air and feed samples from deer farm for isolation and identification of bacteria, bacterial count, bacterial 16S rDNA PCR amplification, sequencing analysis, and the pathogenic bacteria and conditional pathogenic bacteria of drug sensitivity test. The results showed that there were 27 strains of bacteria isolated and purified, enumeration of male and female deer's pellet feed, fodder and water were 1.0×10⁵,8.7×10⁴,2.7×10⁷ CFU/g and 2.9×10⁴ CFU/mL, respectively. Description of water and fodder did not conform to the standard of the national health requirements; 16S rDNA gene sequences comparison in NCBI showed that these bacteria belonged to 15 kinds of different kinds, like Escherichia coli, Shigella sp., and so on. There were 13 kinds of pathogenic bacteria and conditional pathogenic bacteria. The 13 kinds were resistant to furazolidone, polymyxin B and cephalexin, and were sensitive to ceftriaxone, ciprofloxacin and amikacin. These results provided basis for efficient control of the disease in deer farm.

Key words: deer farm; environment; bacterial distribution; drug resistance investigation

CLC Number:

S855.1

WANG Chao, ZHANG Meng-nan, NIE Lin, SHEN Nai-wen, LIU Xin, ZAHNG Yi, HE Jian-bin, YIN Rong-huan. Distribution and Drug Resistance Investigation of Bacteria from Deer Farm Environment[J]. , 2017, 44(3): 904-911.

References

[1] 唐蕊.中国鹿产业发展现状及策略研究[D].长春:吉林大学,2013.
[2] 王艳丽.畜牧业污染现状及对策措施[J].中国畜牧兽医文摘,2015,31(11):14.
[3] Hartmann A,Locatelli A,Amoureux L,et al. Occurrence of CTXM producing Escherichia coli in soils,cattle,and farm environment in France (Burgundy region)[J].Front Microbiol,2012,3:83.
[4] 张鹤平. 养殖场及其周围环境空气中细菌播散研究[D].北京:中国农业大学,2005.
[5] 段会勇. 动物舍微生物气溶胶及其向周围环境的传播[D].泰安:山东农业大学,2008.
[6] Matkovic K,Vucemilo M,Stokovic I,et al. Concentrations of airborne bacteria and fungi in a livestock building with caged laying hens[J].Veterinarski Arhiv,2013,83(4):413-424.
[7] Heederik D,Brouwer R,Biersteker K,et al. Relationship of airborne endotoxin and bacteria in pig farms with the lung function and respiratory symptoms of farmers[J].Int Arch Occup Environ Health,1991,62(8):595-601.
[8] 李世静,嵇辛勤,文明,等.养鸭场主要细菌分离鉴定及致病性研究[J]. 中国畜牧兽医,2014,41(12):267-271.
[9] 徐玉薇,罗小青,魏学良,等.不同地区3个奶牛场临床型乳房炎细菌病原种类分析[J]. 中国畜牧兽医,2016,43(2):548-552.
[10] Jonas D,Rosenbaum A,Weyrich S,et al. Enzyme linked immunoassay for detection of PCR amplified DNA of legionellae in broncho aveolar fluid[J].J Clin Microbiol,1995,33(5):1247-1257.
[11] Matsuki T,Watanabe K,Fujimoto J,et al. Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces[J].Applied Environmental Microbiology,2002,68(11):5445-5451.
[12] Jones A M,Harrison R M. The effects of meteorological factors on atmospheric bioaerosol concentrations——A review[J].Sci Total Environ,2004,326(1-3):151-180.
[13] Vogelzang P F,van der Gulden J W,Folgering H,et al. Endotoxin exposure as a major determinant of lung function deline in pig farmers[J].Am J Rrspir Crit Care Med,1998,157(1):15-18.
[14] Dungan R S.Airborne endotoxin from indoor and outdoor environments:Effect of sample dilution on the kinetic Limulus amebocyte lysate (LAL) assay[J].Journal of Occupational and Environmental Hygiene,2011,8(3):147-153.
[15] Du X J, Zhang X, Li P.et al. Screening of genes involved in interactions with intestinal epithelial cells in Cronobacter sakazakii[J].AMB Express,2016,6(1):74.
[16] 康静静,杨玉荣,梁宏德.志贺氏菌病发病机制的研究进展[J].中国农业科学,2011,44(9):1939-1944.
[17] Pei X Y, Yan L, Zhu J H, et al. The survey of Cronobacter spp. (formerly Enterbacter sakazakii) in infant and follow-up powdered formula China in 2012[J].Biomed Environ Sci,2016,29(2):99-106.
[18] Kuo S M. Gender difference in bacteria endotoxin-induced inflammatory and anorexic responses[J]. PLoS One, 2016,11(9):e0162971.
[19] 刘会胜,赵战勤,薛云,等.羊源致病性弗氏柠檬酸杆菌的分离与鉴定[J].畜牧兽医学报,2015,46(9):1593-1599.
[20] 王克迪,吕治,苏建荣.细菌性阴道病患者阴道菌群特征的非培养分析[J].标记免疫分析与临床,2011,18(6):402-407.
[21] 高正琴,张强,贺争鸣,等.中国小型猪细菌分离鉴定及药敏试验[J].中国人兽共患病学报,2010,26(1):46-52.
[22] 陈必芳,许齐放.GB13078-2001.饲料卫生标准[S].北京:中华人民共和国国家质量监督检验检疫总局,2001.
[23] 向军,张金虎,柴海云,等.甲基营养型芽孢杆菌产氨肽酶的发酵优化[J].中国酿造,2012,31(5):45-50.

Distribution and Drug Resistance Investigation of Bacteria from Deer Farm Environment

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Xiaoyu, LI Ruoyu, WANG Xiao, WANG Lili, LI Jibin, LI Shuying, XU Yongping. Research Progress on the Influence of Filamentous Phages on Host Bacterial Adaptability [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2496-2506.
[2]	ZHAO Qianming, QI Meng, XU Zeli, GONG Pihong, LU Chenyang, PEI Zhiyang, JIAN Fuchun, WANG Jiandong, JING Bo, NING Changsheng. Investigation of Intestinal Parasites Occurrence in Sheep and Environmental Contamination in Large-scale Housing Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2156-2165.
[3]	ZHANG Xiaoke, LIAO Weili, LI Tingting, LI Yao, LI Jiaqi, HUANG Xiang, ZHANG Hao. Effects of Semen Collectors and Environmental Parameters in Pigpen on Semen Quality of Duroc Boars [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 220-229.
[4]	DONG Wanyu, ZHANG Jumin, BIAN Sushu, ZHOU Yingshan, DU Jing, YU Haoran, ZHONG Wei, SONG Houhui, WANG Xiaodu. Comparative Analysis of Environmental Factors in Fattening House Under Two Ventilation Modes in Hangzhou Large-scale Pig Farm [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 421-428.
[5]	YAN Hao, FENG Jianyuan, ZHANG Ziyi, YANG Songxin, XU Chunyu, WEI Meng, HUANG Qingping, CHEN Hailan. Progress in Preparation and Clinical Application of Nanobody [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 685-694.
[6]	LI Ning, ZHANG Yangdong, HUANG Guoxin, LIU Kaizhen, ZHENG Nan, WANG Jiaqi, ZANG Changjiang. Effects of Short-term Addition of High-level Flaxseeds on Dairy Performance, Fatty Acid Profiles of Milk and Rumen Fermentation [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3950-3961.
[7]	LI Pengxing, LI Qing, CHEN Dayong, HE Huijie, JI Xiang, GAO Dong, GAO Aiqin. Comparative Study on Physiological,Serum Biochemical Indexes and Immune Performance of Dorper Sheep,Suffolk Sheep and Mongolian Sheep Under Low Temperature Environment [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 109-116.
[8]	ZHANG Xuejing, HE Zhuolin, HOU Xiao, PU Wanxia. Detection of Resistance and Resistance Genes of Dairy Cow Pastureland Environment in Gansu Province [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1882-1891.
[9]	MA Wenjin, XU Xiangyue, AN Boyu, CHENG Guyue, HUANG Lingli. Research Progress on Environmental Risk Assessment of Veterinary Drugs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1628-1636.
[10]	XU Xiangyue, MA Wenjin, AN Boyu, CHENG Guyue, HUANG Lingli. Advances on Risk Assessment of Tetracycline Antibiotics in the Environment [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 948-957.
[11]	GAO Dengying, LEI Minggang. Effects of High Environmental Temperature on Serum Oxidative Stress Factors and Jejunal Mucosal Mechanical Barrier Function in Tongcheng Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3114-3122.
[12]	JIAO Weina, LI Zhuocheng, LIU Hongjia, YANG Huiguo, HE Zonglin, YAO Gang, WANG Jinquan. Comparative Study on Spring Environmental Indices and Lamb's Health Status of Two Intensive Sheep Farms in South and North of Xinjiang [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 306-314.
[13]	WANG Yuke, OU Yahong, ZHANG Liyun, WANG Yanxin, WANG Xu, PENG Dapeng, WANG Yulian, PAN Yuanhu, XIE Shuyu, CHEN Dongmei, HUANG Lingli, TAO Yanfei. Research Progress on Sample Preparation for Detection of Sulfonamides and Its Metabolites in Animal Food and Environment [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3032-3041.
[14]	LI Peng, ZHANG Yangdong. Research Progress on Analysis Methods for Triazine Herbicides and Their metabolites [J]. , 2018, 45(7): 2025-2033.
[15]	ZHANG Ping, PENG Linyao, XU Changwen, LI Yunxia, ZHANG Anyun. Study on Florfenicol Resistance and fexA Genetic Environment in Enterococcus from Swine [J]. , 2018, 45(6): 1700-1707.