鸭腺病毒A型Hexon基因克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2017.10.030

Abstract

Abstract:

In order to clarify the characteristics of the Hexon gene and the classification candidate of duck adenovirus A under the Adenoviridae family, the Hexon gene fragments of duck adenovirus A (designated as strain JX2016) were amplified. The results demonstrated that the cloned Hexon gene of duck adenovirus A (strain JX2016) was 2 733 bp in length, coding 910 amino acids. Nucleotide homology comparison showed that the strain JX2016 shared the highest nucleotide (99.8%) homology with reference strain FJ12025, also displayed higher than 99.4% nucleotide homology with other duck adenovirus A reference strains. However, the nucleotide homologies with strain GR (duck adenovirus 2, unclassified virus), Phelps (fowl adenovirus A) and P29 (goose adenovirus) were 54.5%, 53.6% and 55.2%, respectively. The genetic evolution analysis showed that all the duck adenovirus A strains were at the same subgroup, under the same Atadenovirus genus branch with ovine atadenovirus D (strain OAV287). Meanwhile, the duck adenovirus 2 (strain GR) at the same branch under Aviadenovirus genus branch with fowl adenovirus A (strain Phelps) and goose adenovirus (strain P29). Based on the Hexon protein genetic evolution analysis, we suggested renamed the duck adenovirus A as duck-origin atadenovirus and renamed the duck adenovirus 2 as duck-origin aviadenovirus.

Key words: duck adenovirus A; Hexon gene; sequence analysis; genetic evolution; classification

CLC Number:

S852.65

WAN Chun-he, LIU Rong-chang, CHEN Cui-teng, CHENG Long-fei, FU Guang-hua, FU Qiu-ling, SHI Shao-hua, CHEN Hong-mei, HUANG Yu. Cloning and Sequence Analysis of Hexon Gene of Duck Adenovirus A[J]. , 2017, 44(10): 3042-3048.

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Cloning and Sequence Analysis of Hexon Gene of Duck Adenovirus A

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