不同卵黄成分、大豆卵磷脂及β-环糊精胆固醇对马精子冷冻效果的影响

doi:10.16431/j.cnki.1671-7236.2016.09.031

Abstract

Abstract:

In the present study,we investigated the effect of different yolk components (whole egg yolk (EY),egg yolk plasma proteins (EYP),low-density lipoprotein (LDL) and livetin),soy lecithin (SL) and cholesterol-loaded cyclodextrin (CLC) on equine sperm cryopreservation.Semen was collected from 4 adult thoroughbred stallions,experiments 1:Adding 0,2%,4% and 8% concentration of EY,LDL,EYP or livetin in INRA82+3.5% combination cryoprotectants;Experiments 2:Adding 1%,2% and 4% SL with 4% EY as control group;Experiment 3:Adding 0,0.75,1.5 and 3 mg/mL concentration of CLC.The results showed that EYP,LDL and livetin had a significant cryoprotective function(P<0.05),but no significant difference was observed between different components of the yolk and whole egg yolk (P>0.05);Addition of 1% SL obtained similar cryopreservation result with the control group (4% egg yolk) (P>0.05),but post-thaw TM,PM and RAP supplemented with 2% and 4% SL group were significantly lower than the control group (P<0.05);There was no significant difference between different concentrations of CLC group and the control group in thawed sperm motility (P>0.05).In conclusion,EYP,LDL and livetin had a significant cryoprotective function,but no significant difference with EY;Addition of 1% SL could obtain similar cryopreservation result with the 4% EY group;Supplying CLC in extender contain egg yolk could not improve post-thaw equine semen quality.

Key words: yolk; low-density lipoprotein (LDL); soy lecithin; cholesterol-loaded cyclodextrin (CLC); horse; sperm cryopreservation

CLC Number:

S821

WU Zhuang-yuan, LUO Yong-ming, DONG Hong, HUO Fei, ZHENG Xin-bao, YU Wei-hao, XU Wen-hui, CHEN Jing-bo. Effect of Different Yolk Composition,Soybean Lecithin and Cholesterol-loaded Cyclodextrin on Equine Sperm Cryopreservation[J]. , 2016, 43(9): 2435-2440.

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Effect of Different Yolk Composition,Soybean Lecithin and Cholesterol-loaded Cyclodextrin on Equine Sperm Cryopreservation

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