牛源大肠杆菌Ⅰ类整合子分子特征及其与耐药性关系的研究

Abstract

Abstract: To investigate the prevalence and molecular characterization of class Ⅰ integron in Escherichia coli isolated from beef cattle, and analyze the relationship between integron and antimicrobial resistance, susceptibilities to 11 antimicrobials were conducted on 92 isolates, the presence and characterization of class Ⅰ integrons and inserted gene cassettes were performed using PCR combined with sequencing analysis. The results showed that 29 isolates had been detected positive for class Ⅰ integron integrase gene (intⅠ1) among 92 isolates, and aadA1 and dfrA17＋aadA5 were the most prevalent gene cassette arrays detected. The resistance rates of 92 isolates to ampicillin, streptomycin, sulfisoxazole and tetracycline were all more than 45.0%. As revealed by analyzing the association between resistance phenotypes and class Ⅰ integron, isolates that contained the class Ⅰ integron were significantly highly resistant to ampicillin, chloramphenicol, streptomycin, sulfisoxazole and sulfamethoxazole-trimethoprim (P<0.05), but not to quinolones and amoxicillin/clavulanic acid. The conclusion was that E.coli isolated from beef cattle were seriously resistant to antimicrobials,and integron/cassettes widely existed. The presence of integrons and the association of antimicrobial resistance determinants with transferable elements might play a crucial role in the dissemination of antibiotic resistance among E.coli. Data reported here clearly emphasized the need for a stricter application of antimicrobials restriction policies in feedlot setting.

Key words: class Ⅰ integron; gene cassettes; antimicrobial resistance; Escherichia coli

CLC Number:

S852.61^＋4

WANG Hai-sheng, HU Rui-ping, GAO Yu-min, YANG Zhong, DENG Xiu-ling, ZHANG Jian-yu, WU Rui-bing, HUHE Bateer. Study on Molecular Characterization of Class Ⅰ Integron and Integron-associated Antimicrobial Resistance in Escherichia coli from Beef Cattle[J]. , 2014, 41(9): 63-68.

References

1 Barlow R S, Fegan N, Gobius K S. Integron-containing bacteria in faeces of cattle from different production systems at slaughter[J]. J Appl Microbiol, 2009, 107(2): 540～545.

2 Cambray G, Guerout A, Mazel D. Integron[J]. Annu Rev Genet, 2010, 44: 141～166.

3 Cameron F, Groot Obbink D, Ackerman V, et al. Evolutionary relationship of this region with those surrounding aadA in R538-1 and dhfrⅡ in R388[J]. Nucl Acids Res, 1986, 14: 8625～8635.

4 Chen B, Zheng W, Yu Y, et al. Class 1 integrons, selected virulence genes, and antibiotic resistance in Escherichia coli isolates from the Minjiang river, Fujian province, China[J]. Appl Environ Microbiol, 2011,77(1): 148～155.

5 Hall R M. Integrons and gene cassettes: Hotspots of diversity in bacterial genomes[J]. Ann N Y Acad Sci, 2012, 1267: 71～78.

6 Kadlec K, Schwarz S. Analysis and distribution of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected in the BfT-GermVet monitoring study[J]. J Antimicrob Chemother, 2008, 62(3): 469～473.

7 Kang H Y, Jeong Y S, Oh J Y, et al. Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea[J]. J Antimicrob Chemother, 2005, 55(5): 639～644.

8 Mazel D, Dychinco B, Webb V, et al. Antibiotic resistance in the ECOR collection: Integrons and identification of a novel aad gene[J]. Antimicrob Agents Chemother, 2000, 44: 1568～1574.

9 Mazel D. Integrons: Agents of bacterial evolution[J]. Nat Rev Microbiol, 2006, 4: 608～620.

10 Ozgumus O B, Sandalli C, Sevim A, et al. Class 1 and class 2 integrons and plasmid-mediated antibiotic resistance in coliforms isolated from ten rivers in northern Turkey[J]. J Microbiol, 2009, 47(1): 19～27.

11 Recchia G D, Hall R M. Gene cassettes: A new class of mobile element[J]. Microbiology, 1995, 141(Pt12): 3015～3027.

12 Sáenz Y, Vinue L, Ruiz E, et al. Class 1 integrons lacking qacEDelta1 and sul1 genes in Escherichia coli isolates of food, animal and human origins[J]. Vet Microbiol, 2010, 144(3～4): 493～497.

13 Soufi L, Abbassi M S, Saenz Y, et al. Prevalence and diversity of integrons and associated resistance genes in Escherichia coli isolates from poultry meat in Tunisia[J]. Foodborne Pathog Dis, 2009, 6(9): 1067～1073.

14 Stokes H W, O'Gorman D B, Recchia G D, et al. Structure and function of 59-base element recombination sites associated with mobile gene cassettes[J]. Mol Microbiol, 1997, 26(4): 731～745.

15 Vinué L, Saenz Y, Somalo S, et al. Prevalence and diversity of integrons and associated resistance genes in faecal Escherichia coli isolates of healthy humans in Spain[J]. J Antimicrob Chemother, 2008, 62(5): 934～937.

[1]	YUAN Jingzhi, YANG Liyao, CHEN Siyu, CAI Zongling, LI Xun, LIAO Yuying, WANG Xiaoye. Investigation of Antimicrobial Resistance and Antimicrobial Resistance Genes of E.coli from the Live Poultry Production Chain [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2661-2670.
[2]	YUAN Xiaomeng, ZHAO Xiaonan, LI Lulu, ZHANG Qing, HU Ming, LUO Yanbo, ZHANG Yin, QI Jing, LIU Yuqing. Isolation,Identification and Drug Resistance Analysis of E.coli from Mastitis Milk in Shandong Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 312-323.
[3]	CEN Daoji, MAI Jialin, ZHOU Yuyuan, GENG Haoyu, LIU Changhai, DENG Guohui, JIANG Qi, FANG Liangxing. Characteristics of the Antimicrobial Resistance and Dissemination of bla_CTX-M Genes Among E.coli from Geese Farms in Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1571-1582.
[4]	YU Zhongna, JIA Lingyun, ZHEN Tianyuan, WANG Jun, HAN Rongwei. Analysis of Epidemicity and Antimicrobial Resistance of Staphylococcus aureus Isolated from Mastitis Milk in Xinjiang and Inner Mongolia [J]. , 2019, 46(4): 1199-1208.
[5]	WANG Dixuan, GAO Dongyang, ZHANG Tianzi, LIU Wei, ZHOU Zutao, XU Xiaojuan, CAI Xuwang. Isolation,Identification and Drug Resistance Analysis of Avian Salmonella in Central China [J]. , 2019, 46(3): 931-939.
[6]	ZHANG Mingyang, HU Andong, CHENG Zhentao, JIANG Haibo, WEN Ming. Isolation,Identification and Drug Resistance Analysis of Plesiomonas shigelloides from Hybrid Surgeon [J]. , 2019, 46(1): 264-270.
[7]	QIAN Xiao-lu, SHANG Jun, TIAN Kai, ZHANG Hao-ran. Analysis of Ribosome Genotyping and Antimicrobial Resistance in Enterococcus faecalis Isolated from Pigs and Chickens [J]. , 2016, 43(8): 2176-2182.
[8]	ZHAO Jun-li, SHI Rui-li, LI Zhi-fang, DING Yue-xia, LI Pei-feng, GUAN Hong. Analysis of Antimicrobial Resistance and Detection of ESBLs Genes of Pathogenic Salmonella Isolates from Dairy Cows in Inner Mongolia [J]. , 2015, 42(8): 2150-2159.
[9]	YANG Xiao-wei，KUANG Dai，PAN Hai-jian，SU Xu-dong，WU Li-ping，ZHANG Jian-min. Antimicrobial Resistance and Pulse Field Gel Electrophoresis Analysis of Salmonella agona Strains from Poultry in Shanghai [J]. , 2014, 41(2): 213-217.
[10]	GUO Xiao-mu，YANG Tong，HE Dan-dan，RAO Li-li，LIU Jian-hua，ZENG Zhen-ling. Antimicrobial Resistance and Phylogenetic Analysis of E.coli Isolated from Swine in Guangdong Province [J]. , 2014, 41(1): 182-186.
[11]	GAO Lu-yan, YANG Zhong, WU Rui-bing, ZHENG Wu-jiang. Research Progresss on Bacterial Antimicrobial Resistance [J]. , 2013, 40(7): 162-166.
[12]	SU Yang, PU Wan-xia, DENG Hai-ping, LI Chun-hui, LIANG Hong-yan, CHEN Zhi-hua. Detection of Methicillin-resistant Staphylococcus aureus Strains Isolated from Bovine Mastitis [J]. , 2013, 40(3): 181-184.
[13]	JIN Xin;SU Rui;ZHANG Wen-guang;ZHANG Yan-jun;WANG Rui-jun;LI Jin-quan. Analysis on Antimicrobial Susceptibility to Tetracycline Analogues for Three Genotypes of Tetracycline Resistance in Escherichia coli Isolates from Feedlot Cattle [J]. , 2012, 39(5): 187-191.
[14]	JIN Xin, ZHANG Wen-guang, ZHANG Yan-jun, SU Rui, WANG Rui-jun, LI Jin-quan. Abundance and Persistence of Tetracycline Resistance Genes in Fecal Depositsy from Beef Cattle Fed Different Subtherapeutic Antibiotics [J]. , 2012, 39(10): 101-105.
[15]	ZHANG Miao-miao;DAI Meng-hong;HUANG Ling-li;WANG Yu-lian;YUAN Zong-hui. Canadian Administration on Veterinary Antimicrobial Resistance [J]. , 2011, 38(12): 218-221.

Study on Molecular Characterization of Class Ⅰ Integron and Integron-associated Antimicrobial Resistance in Escherichia coli from Beef Cattle

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments