蛋白质组学研究方法及应用的研究进展

Abstract

Abstract: Proteins are the chief performers for physiological functions in a cell, and often function as protein complexes or interact with nucleic acids. A proteome represents all the proteins expressed by a cell, a tissue, or an organism at a defined time point under certain circumstances. Therefore, proteomes will undergo dynamic changes with alterations in time and space. The aim of proteomics is to globally study structures, functions and expression modes of proteome as well as interactions among proteins in a proteome. Based on the contents of study, proteomics can be classified as expression proteomics, structural proteomics and functional proteomics,respectively. The study of proteomes will help understand structures of proteins, functions of cells, nature and active rules of life, and will provide scientific basis for diagnosis and treatment of diseases as well as development of novel drugs. The techniques commonly used in proteomic studies include two-dimentional polyacrylamide gel electrophoresis, mass spectrometry, yeast two-hybrid system and protein chips. This article will provide a brief overview of proteomics and techniques frequently utilized for proteomic studies.

Key words: proteomics; two-dimentional gel electrophoresis; mass spectrometry; protein analysis

CLC Number:

TAN Wei, HUANG Li, XIE Zhi-xun. Research Progress on Proteomic Techniques and their Applications[J]. , 2014, 41(9): 40-46.

References

1 孙成友,李娟,刘雨田,等. 蛋白质组学技术在病毒及其感染机制研究中的应用[J]. 中国畜牧兽医,2013,40(5):75～79.

2 张太翔,田国宁,张金玲,等. 蛋白质组学与神经系统疾病的研究进展[J]. 中国畜牧兽医,2008,35(7):46～48.

3 党源. 新城疫病毒单感染及猪流感病毒与猪链球菌共感染差异蛋白质组学分析[D]. 长春:吉林大学,2013.

4 高昭辉,董书伟,薛慧文,等. 纳米铜对大鼠肝脏毒性的蛋白质组2-DE图谱分析[J]. 中国畜牧兽医,2012,39(11):22～26.

5 Abel L, Kutschki S, Turewicz M, et al. Autoimmune profiling with protein microarrays in clinical applications[J]. Biochim Biophys Acta, 2014, 1844(5): 977～987.

6 Altelaar A F, Munoz J, Heck A J. Next-generation proteomics: Towards an integrative view of proteome dynamics[J]. Nat Rev Genet, 2013, 14(1): 35～48.

7 Alvarez-Chaver P, Otero-Estévez O, Páez de la Cadena M, et al. Proteomics for discovery of candidate colorectal cancer biomarkers[J]. World J Gastroenterol, 2014, 20(14): 3804～3824.

8 Andersen J S, Mann M. Functional genomics by mass spectrometry[J]. FEBS Lett, 2000, 480(1): 25～31.

9 Anderson L, Seilhamer J. A comparison of selected mRNA and protein abundances in human liver[J]. Electrophoresis, 1997, 18(3～4): 533～537.

10 Arenkov P, Kukhtin A, Gemmell A, et al. Protein microchips: Use for immunoassay and enzymatic reactions[J]. Anal Biochem, 2000, 278(2): 123～131.

11 Banks R E, Dunn M J, Hochstrasser D F, et al. Proteomics: New perspectives, new biomedical opportunities[J]. Lancet, 2000, 356(9243): 1749～1756.

12 Beck M, Claassen M, Aebersold R. Comprehensive proteomics[J]. Curr Opin Biotechnol, 2011, 22(1): 3～8.

13 Brewis I A, Brennan P. Proteomics technologies for the global identification and quantification of proteins[J]. Adv Protein Chem Struct Biol, 2010, 80: 1～44.

14 Cho A, Normile D. Nobel prize in chemistry. Mastering macromolecules[J]. Science, 2002, 298(5593): 527～528.

15 Cho W C. Proteomics technologies and challenges[J]. Genomics Proteomics Bioinformatics, 2007, 5(2): 77～85.

16 de Hoog C L, Mann M. Proteomics[J]. Annu Rev Genomics Hum Genet, 2004, 5: 267～293.

17 Ding S J, Qian W J, Smith R D. Quantitative proteomic approaches for studying phosphotyrosine signaling[J]. Expert Rev Proteomics, 2007, 4(1): 13～23.

18 Domon B, Aebersold R. Mass spectrometry and protein analysis[J]. Science, 2006, 312(5771): 212～217.

19 Fenn J B, Mann M, Meng C K, et al. Electrospray ionization for mass spectrometry of large biomolecules[J]. Science,1989, 246(4926): 64～71.

20 Fields S, Song O. A novel genetic system to detect protein-protein interactions[J]. Nature, 1989, 340(6230): 245～246.

21 Godovac-Zimmermann J, Brown L R. Perspectives for mass spectrometry and functional proteomics[J]. Mass Spectrom Rev, 2001, 20(1): 1～57.

22 Graves P R, Haystead T A. Molecular biologist's guide to proteomics[J]. Microbiol Mol Biol Rev, 2002, 66(1): 39～63.

23 Gygi S P, Rochon Y, Franza B R, et al. Correlation between protein and mRNA abundance in yeast[J]. Mol Cell Biol, 1999, 19(3): 1720～1730.

24 Gygi S P, Corthals G L, Zhang Y, et al. Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology[J]. Proc Natl Acad Sci USA, 2000, 97(17): 9390～9395.

25 Hanash S. Disease proteomics[J]. Nature, 2003, 422(6928): 226～232.

26 Hendrickson R C, Douglass J F, Reynolds L D, et al. Mass spectrometric identification of mtb81, a novel serological marker for tuberculosis[J]. J Clin Microbiol, 2000, 38(6): 2354～2361.

27 Karas M, Hillenkamp F. Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons[J]. Anal Chem, 1988, 60(20): 2299～2301.

28 Klose J. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals[J]. Humangenetik, 1975, 26(3): 231～243.

29 Kopf E, Zharhary D. Antibody arrays——An emerging tool in cancer proteomics[J]. Int J Biochem Cell Biol, 2007, 39(7～8): 1305～1317.

30 Krishna R G, Wold F. Post-translational modification of proteins[J]. Adv Enzymol Relat Areas Mol Biol, 1993, 67: 265～298.

31 MacBeath G, Schreiber S L. Printing proteins as microarrays for high-throughput function determination[J]. Science, 2000, 289(5485): 1760～1763.

32 Maxwell K L, Frappier L. Viral proteomics[J]. Microbiol Mol Biol Rev, 2007, 71(2): 398～411.

33 May C, Brosseron F, Chartowski P, et al. Instruments and methods in proteomics[J]. Methods Mol Biol, 2011, 696: 3～26.

34 McKerrow J H, Bhargava V, Hansell E, et al. A functional proteomics screen of proteases in colorectal carcinoma[J]. Mol Med, 2000, 6(5): 450～460.

35 Nishimura T, Ogiwara A, Fujii K, et al. Disease proteomics toward bedside reality[J]. J Gastroenterol, 2005, 40(Suppl)16: 7～13.

36 O'Donovan C, Apweiler R, Bairoch A. The human proteomics initiative (HPI)[J]. Trends Biotechnol, 2001, 19(5): 178～181.

37 O'Farrell P H. High resolution two-dimensional electrophoresis of proteins[J]. J Biol Chem, 1975, 250(10): 4007～4021.

38 Patton W F. Detection technologies in proteome analysis[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2002, 771(1～2): 3～31.

39 Paweletz C P, Charboneau L, Bichsel V E, et al. Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front[J]. Oncogene, 2001, 20(16): 1981～1989.

40 Petschnigg J, Snider J, Stagljar I. Interactive proteomics research technologies: Recent applications and advances[J]. Curr Opin Biotechnol, 2011, 22(1): 50～58.

41 Pratsch K, Wellhausen R, Seitz H. Advances in the quantification of protein microarrays[J]. Curr Opin Chem Biol, 2014, 18: 16～20.

42 Rao V S, Srinivas K, Sujini G N, et al. Protein-protein interaction detection: Methods and analysis[J]. Int J Proteomics, 2014, 2014: 147648.

43 Rappsilber J, Mann M. What does it mean to identify a protein in proteomics[J]. Trends Biochem Sci, 2002, 27(2): 74～78.

44 Robinson W H, DiGennaro C, Hueber W, et al. Autoantigen microarrays for multiplex characterization of autoantibody responses[J]. Nat Med, 2002, 8(3): 295～301.

45 Rodrigo M A, Zitka O, Krizkova S, et al. MALDI-TOF MS as evolving cancer diagnostic tool: A review[J]. J Pharm Biomed Anal, 2014, 95: 245～255.

46 Scheele G A. Two-dimensional gel analysis of soluble proteins. Charaterization of guinea pig exocrine pancreatic proteins[J]. J Biol Chem, 1975, 250(14): 5375～5385.

47 Snider J, Kittanakom S, Damjanovic D, et al. Detecting interactions with membrane proteins using a membrane two-hybrid assay in yeast[J]. Nat Protoc, 2010, 5(7): 1281～1293.

48 Thongboonkerd V. Proteomics[J]. Forum Nutr, 2007, 60: 80～90.

49 Uetz P, Giot L, Cagney G, et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae[J]. Nature, 2000, 403(6770): 623～627.

50 Unlü M, Morgan M E, Minden J S. Difference gel electrophoresis: A single gel method for detecting changes in protein extracts[J]. Electrophoresis, 1997, 18(11): 2071～2077.

51 Wasinger V C, Cordwell S J, Cerpa-Poljak A, et al. Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium[J]. Electrophoresis, 1995, 16(7): 1090～1094.

52 Weinrich D, Jonkheijm P, Niemeyer C M, et al. Applications of protein biochips in biomedical and biotechnological research[J]. Angew Chem Int Ed Engl, 2009, 48(42): 7744～7751.

53 Wilkins M R, Sanchez J C, Gooley A A, et al. Progress with proteome projects: Why all proteins expressed by a genome should be identified and how to do it[J]. Biotechnol Genet Eng Rev, 1996, 13: 19～50.

54 Xiao Z, Jiang X, Beckett M L, et al. Generation of a baculovirus recombinant prostate-specific membrane antigen and its use in the development of a novel protein biochip quantitative immunoassay[J]. Protein Expr Purif, 2000, 19(1): 12～21.

55 Zhu H, Bilgin M, Bangham R, et al. Global analysis of protein activities using proteome chips[J]. Science, 2001, 293(5537): 2101～2105.

[1]	MA Jifu, BAKHET·Bodai, KEZERBEK·Kazibek, LI Zhenwei, ORALHAZI·Hazikhan. Screening and Identification of Inhibin α Subunit-specific Nanobody Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1532-1542.
[2]	WU Tao, QI Yunxia, LIU Wei, ZHU Honglong, HUANG Dongwei, DING Haisheng, ZHAO Huiling, CHENG Guanglong, ZHAO Xiaowei. Effect of Colostrum and Milk Feeding on Hepatic Proteins of Calves Based on Proteomics Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 37-45.
[3]	DAI Guodong, YAN Hongbin, LI Li, FU Baoquan, JIA Wanzhong. Research Progress on Screening Methods for Candidate Vaccine Molecules Against Parasitic Zoonoses [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2177-2187.
[4]	ZHU Lan, WANG Peng, HONG Qionghua, YANG Hongyuan, ZHAO Xiaohong, LAN Rong. Association Analysis of Three SNPs on Chromosome 28 with Lambing Number in Yunshang Black Goat [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1294-1301.
[5]	HE Dongqian, TAO Jinzhong, CHEN Liyao, WANG Junkui. Research Progress of Sheep Hair Follicle and Keratin [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 248-256.
[6]	ZHENG Xingguo, GUO Lingan, ZHAO Shan, LI Xi, LEI Xinyu, ZHONG Lingli. Determination of Robenidine and Its Metabolites in Animal Derived Food by UPLC-MS/MS [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1961-1970.
[7]	NIU Chunyu, SHI Linlin, SUN Miao, LIU Yanmin, HUANG Shaolei, CHENG Chao, LI Peifeng. Applications of Omics Approaches in Isolation and Identification of Lactic Acid Bacteria and It’s Functional Research [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3882-3889.
[8]	WANG Xinyue, ZHANG Li. Research Progress on Application of Proteomics and Post-translational Modification of Proteins in Animal Husbandry [J]. , 2019, 46(4): 1063-1073.
[9]	ZHONG Lingli, GUO Lingan, MAO Jianfei, LI Xi, HU Li, LEI Xinyu, OUYANG Huaxue, LI Huaxian. Simultaneous Determination of Tilmicosin, Dexamethasone, Florfenicol and Chloramphenicol Residues in Milk by UPLC-MS/MS Method [J]. , 2018, 45(9): 2645-2652.
[10]	WU Kainian, WANG Li, LI Chenyang. Cloning and Bioinformatics Analysis of BCL2A Gene in Jinchuan Yak [J]. , 2018, 45(6): 1463-1470.
[11]	SU Jun, LIU Man, WANG Jigui, SUN Yan, LIU Weiquan. Prokaryotic Expression and Specific Antiserum Preparation of Mink Growth Hormone [J]. , 2018, 45(4): 866-872.
[12]	DUAN Hui-qin, WANG Li, LI Jian, XIONG Xian-rong. Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak [J]. , 2016, 43(6): 1430-1436.
[13]	FU Xue-feng, YANG Han-yulu, SHI Gang, XU Xin-ming, ZHANG En-ping, DI Jiang, ZHANG Yan-hua, HUANG Xi-xia, TIAN Ke-chuan. Proteome Array on Differentially Expressed Proteins of Skin Tissue in Fine-wool Sheep with Different Fiber Diameter [J]. , 2016, 43(4): 879-891.
[14]	CHANG Ping-ping, XU Chun-nan, ZHANG Li-xin, HAO Xiao-mei, GAO Xing, CHEN Chao. Establishment of Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Determination Method of Amantadine in Chicken and Preparation of Positive Samples [J]. , 2015, 42(9): 2278-2285.
[15]	DENG Ting-xian, PANG Chun-ying, ZHU Peng, DUAN An-qin, LU Xing-rong, YANG Bing-zhuang, LIANG Xian-wei. Rapid Detection of SNPs in Buffalo MC4R Gene by Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry [J]. , 2015, 42(7): 1800-1806.

Research Progress on Proteomic Techniques and their Applications

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments