脂肪酸合成酶基因mRNA在马身猪和大白猪3种组织中的发育性表达研究

Abstract

Abstract: The developmental expressions of fatty acid synthase (FAS) gene mRNA in liver,longissimus dorsi and backfat at seven phases (1,30,60,90,120,150 and 180-day old) of Mashen pig and Large White pig were studied by Real-time quantitative PCR,so that to discuss the spatial and temporal characteristics of FAS gene expression and the relationship with fat metabolism. The results showed that in terms of inter-breeds comparison,the different expression of FAS gene mRNA in liver,longissimus dorsi and backfat of developmental phases of Mashen pig and Large White pig was significant or extremely significant (except in liver at 1-day old and in backfat at 120-day old) (P<0.05;P<0.01). The developmental expressions of FAS gene mRNA in three tissues of Large White pig were age-dependent,during the phases of 150-day and 180-day,the expressions of FAS gene mRNA in backfat was significantly higher than those in liver and longissimus dorsi (P<0.01),and during the phases of 1-day,30-day and 90-day,the expressions of FAS gene mRNA in longissimus dorsi were significantly higher than those in liver and backfat (no samples of 1-day in backfat)(P<0.01). While during the entire developmental phases of Mashen pig,longissimus dorsi was superiority tissue that the expression of the gene was significantly higher than those in other tissues,followed by the expression in backfat,and that in liver was the least. Breed,age,as well as the interactions of breed×age and tissue×age had significant effects on the relative expression of FAS gene mRNA (P<0.01). FAS gene is important for the genetic improvement of meat quality traits of pig as it directly participating in the synthesis of fatty acids.

Key words: pig; FAS gene mRNA; Real-time quantitative PCR; developmental expression

CLC Number:

S828.2

MA Li, GAO Peng-fei, SHI Jian-zhong, NIU Jiao-yan, LI Wei, ZHANG Yan-qing, XU Kai, WANG Ze-yi, LIU Hong, GUO Xiao-hong, LI Bu-gao, CAO Guo-qing. Study on Developmental Expression of FAS Gene mRNA in Three Tissues of Mashen Pig and Large White Pig[J]. , 2014, 41(10): 198-204.

References

1 卢建雄,杨公社,陈粉粉. 胰岛素对原代培养猪脂肪细胞生脂和脂解相关基因转录表达及脂代谢的影响[J]. 农业生物技术学报,2006,14(1):11~16.
2 乔永. 湖羊羔羊不同部位肌肉肌内脂肪沉积相关基因表达的发育性变化研究[D]. 南京:南京农业大学,2007.
3 孟冬梅,王继文,李亚峰. 脂肪酸合成酶基因的研究进展[J]. 上海畜牧兽医通讯,2004,1:12~14.
4 单体中,汪以真,刘建新,等. 不同日龄猪腹脂中脂肪酸合成酶(FAS)基因表达规律的研究[J]. 畜牧兽医学报,2006,37(7):662~666.
5 杨明华,李永能,黄英,等. 日粮蛋白质水平对乌金猪肝脏组织脂类代谢相关基因表达的影响[J]. 中国畜牧兽医,2013,40(2):108~112.
6 鲁凯.北京鸭与黑鹜鸭屠宰性状及脂质沉积相关基因表达差异研究[D]. 雅安:四川农业大学,2013.
7 Abu-Elheiga L,Brinkley W R,Zhong L,et al. The subcellular localization of acetyl-CoA carboxylase 2[J]. Proceedings of the National Academy of Sciences,2000,97(4):1444~1449.
8 Bertil F,Raelot T. mRNA levels of SREBP-1c do not coincide with the changes in adipose lipogenic gene expression[J]. Biochemical and Biophysical Research Communications,2004,325:827~834.
9 Bonnet M,Faulconnier Y,Flechet J,et al. Messenger RNAs encoding lipoprotein lipase,fatty acid synthase and hormone sensitive lipase in the adipose tissue of underfed-refed ewes and cows[J]. Reprod Nutr Dev,1998,38(3):297~307.
10 Chakravarthy M V,Pan Z,Zhu Y,et al. “New” hepatic fat activates PPARα to maintain glucose,lipid,and cholesterol homeostasis[J]. Cell Metabolism,2005,1(5):309~322.
11 Chalbos D,Galtier F,Emiliani S,et al. The anti-progestin RU486 stabilizes the progestin-induced fatty acid synthetase mRNA but does not stimulate its transcription[J]. J Biol Chem,1991,266(13):8220~8224.
12 Chirala S S,Chang H,Matzuk M,et al. Fatty acid synthesis is essential in embryonic development:Fatty acid synthase null mutants and most of the heterozygotes die in utero[J]. Proceedings of the National Academy of Sciences,2003,100(11):6358~6363.
13 Chirala S S,Wakil S J. Structure and function of animal fatty acid synthase [J]. Lipids,2004,39(11):1045~1053.
14 Clay F S. Regulation of fatty acid synthase (FAS) [J]. Prog Lipid Res,1997,36:43~53.
15 Dervishi E,Serrano C,Joy M,et al. The effect of feeding system in the expression of genes related with fat metabolism in semitendinous muscle in sheep[J]. Meat Science,2011,89 (1):91~97.
16 Ding S T,Schinckel A P,Weber T E,et al. Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations[J]. Journal of Animal Science,2000,78(8):2127~2134.
17 Iritani N. Nutritional and hormonal regulation of lipogenic-enzyme gene expression in rat liver[J]. Eur J Biochem,1992,205:433~442.
18 Kim T S,Freake H C. High carbohydrate diet and starvation regulate lipogenic mRNA in rats in a tissue specific manner[J]. Journal of Nutrition,1996,126(3):611~617.
19 Kim K H. Regulation of mammalian acetyl-coenzyme A carboxylase[J]. Annual Review of Nutrition,1997,17(1):77~99.
20 Loftus T M,Jaworsky D E,Frehywot G L,et al. Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors [J]. Science,2000,288(5475):2379~2381.
21 McNeel R L,Ding S T,Smith E O,et al. Expression of porcine adipocyte transcripts during differentiation in vitro and in vivo[J]. Comp Biochem Physiol B Biochem Mol Biol,2000a,126(3):291~302.
22 McNeel R L,Ding S T,Smith E O,et al. Effect of feed restriction on adipose tissue transcript concentrations in genetically lean and obese pigs[J]. Journal of Animal Science,2000b,78(4):934~942.
23 Mildner A M,Clarke S D. Porcine fatty acid synthase:Cloning of a complementary DNA,tissue distribution of its mRNA and suppression of expression by somatotropin and dietary protein[J]. Nutrition and Gene Expression,1991,121:900~907.
24 Moustaid N,Sul H S. Regulation of expression of the fatty acid synthase gene in 3T3-Ll cells by differentiation and triiodothyronine[J]. J Biol Chem,1991,266(28):18550~18554.
25 Nadeau K J,Ehlers L B,Aguirre L E,et al. Exercise training and calorie restriction increase SREBP-1 expression and intramuscular triglyceride in skeletal muscle[J]. American Journal of Physiological Endocrinology Metabolism,2006,291(1):E90~E98.
26 Penicaud L,Ferre P. Increased gene expression of lipogenic enzymes and glucose transporter in white adipose tissue of suckling and weaned obese Zucker rats[J]. Biochem J,1991,279(1):303~308.
27 Qiao Y,Huang Z G,Li Q F,et al. Developmental changes of the FAS and HSL mRNA expression and their effects on the content of intramuscular fat in Kazak and Xinjiang sheep[J]. Journal of Genetics and Genomics,2007,34(10):909~917.
28 Semenkovich C F,Coleman T,Fiedorek F T Jr. Human fatty acid synthase mRNA:Tissue distribution,genetic mapping,and kinetics of decay after glucose deprivation[J]. Journal of Lipid Research,1995,36(7):1507~1521.
29 Soncini M,Yet S F,Moon Y. Hormonal and nutritional control of the fatty acid synthase promoter in transgenic mice[J]. J Biol Chem,1995,270(51):30339~30343.
30 Tian J,Wen H,Zeng L B,et al. Changes in the activities and mRNA expression levels of lipoprotein lipase (LPL),and hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) of Nile tilapia (Oreochromis niloticus) during fasting and refeeding[J]. Aquaculture,2013(400~401):29~35.
31 Tsiplakoua E,Flemetakisb E,Kalloniati C. Differences in mRNA lipogenic gene expression in the subcutaneous adipose tissue of sheep and goats under the same dietary treatments[J]. Small Ruminant Research,2011,99 (2):110~115.
32 Xu Z X,Stenzel W,Sasic S M,et al. Glucocorticoid regulation of fatty acid synthase gene expression in fetal rat lung[J]. Am J Physiol,1993,265:L140~L147.

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Study on Developmental Expression of FAS Gene mRNA in Three Tissues of Mashen Pig and Large White Pig

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