实验猴粪便样品中沙门氏菌LAMP检测方法的建立及应用

Abstract

Abstract: A loop-mediated isothermal amplification (LAMP) assay was developed for detection of Salmonlla in fecal samples of experimental monkeys. According to the specific sequences (fimY) of Salmonlla in GenBank, one set of primers was selected and the reaction condition was optimized. The results showed that the detection limit of LAMP method was 1.35×10¹ and 1.35×10³ CFU/mL in Salmonella pure culture and clinical samples,respectively,which was the same as routine PCR. The amplification could complete in one hour, and the result could be distinguished by naked eyes. There was non-specific amplification of other pathogens. These results suggested that this LAMP assay was a simple and specific method for rapid detection of Salmonlla in fecal samples.

Key words: Salmomella; LAMP; fecal samples; experimental monkeys

QIU Suo-ping, LIN Zhi-xiong, YOU Yong-lai, MA Bao-hua, ZHAO Qiong. Development and Application of Loop-mediated Isothermal Amplification Assay for Salmonella Detection in Fecal Samples of Experimental Monkeys[J]. .

References

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Development and Application of Loop-mediated Isothermal Amplification Assay for Salmonella Detection in Fecal Samples of Experimental Monkeys

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