牛副流感3型病毒Nano-PCR、LAMP方法的建立及初步应用

doi:10.16431/j.cnki.1671-7236.2017.10.002

Abstract

Abstract:

This study was aimed to establish Nano-PCR and LAMP which were new rapid type of molecular detection technologies of bovine parainfluenza type-3 virus (BPIV3).The comparative specificity and sensitivity of BPIV3 Nano-PCR and LAMP PCR were tested, and the assay was applied to detect 10 clinical samples. The specificity and sensitivity tests showed that Nano-PCR and LAMP were only sensitive to BPIV3,without cross reaction to other viruses, such as bovine respiratory syncytial virus,infectious bovine rhinotracheitis virus and bovine viral diarrhea virus. In the sensitivity test, Nano-PCR and LAMP showed 10 times sensitivity than that of the traditional PCR technology,with the minimum detection of 4.16×10² copies/μL. Clinical test results showed that the coincidence rate of Nano-PCR and LAMP could reach to 100%, and the positive detection rate was much higher than that of normal PCR.Therefore, the Nano-PCR and LAMP methods established in this study provided a faster, sensitive and reliable tool for the clinical diagnosis of BPIV3.

Key words: bovine parainfluenza type-3 virus (BPIV3); Nano-PCR; LAMP

CLC Number:

S858.23

LI Jian-you, LI Jia-wei, WANG Chao, GUO Li, HE Hong-bin. Establishment and Preliminary Application of Nano-PCR and LAMP Methods for Bovine Parainfluenza Type-3 Virus[J]. , 2017, 44(10): 2837-2844.

References

[1] Stott E J, Taylor G. Respiratory syncytial virus[J]. Archives of Virology, 1985, 84(1-2):1-52.
[2] 朱远茂,马磊,杨婷,等. 牛副流感的流行、危害及其防控[J]. 中国预防兽医学报,2016,38(8):667-671.
[3] Shibuta H, Kanda T, Adachi A, et al. Characterization of bovine parainfluenza virus type 3[J]. Microbiology and Immunology, 1979, 23(7):617-628.
[4] 冉旭华, 张峣, 曹思, 等. C基因型牛副流感3型病毒的分离鉴定[J]. 中国畜牧兽医, 2016, 43(5):1368-1373.
[5] Oem J K, Lee E Y, Lee K K, et al. Molecular characterization of a Korean bovine parainfluenza virus type 3 isolate[J]. Veterinary Microbiology, 2013, 162(1):224-227.
[6] Griffin D, Chengappa M M, Kuszak J, et al. Bacterial pathogens of the bovine respiratory disease complex[J]. Veterinary Clinics of North America:Food Animal Practice, 2010, 26(2):381-394.
[7] 宋剑白. 牛主要呼吸道病毒病研究进展[J]. 新农村(黑龙江), 2016,12:97.
[8] Schmidt A C, McAuliffe J M, Huang A, et al. Bovine parainfluenza virus type 3(BPIV3) fusion and hemagglutinin-neuraminidase glycoproteins make an important contribution to the restricted replication of BPIV3 in primates[J]. Journal of Virology, 2000, 74(19):8922-8929.
[9] Vaucher R D A, Simonetti A B, Roehe P M. RT-PCR for detection of bovine parainfluenza virus type 3(BPIV-3)[J]. Acta Scientiae Veterinariae, 2008, 36(3):215-220.
[10] 徐子晟,朱远茂. 我国牛副流感病毒3型的研究进展[J]. 中国奶牛,2016, 4:32-34.
[11] Willoughby K, Thomson K, Maley M, et al. Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing[J]. Veterinary Microbiology, 2008, 126(1):264-270.
[12] Seetang-Nun Y, Jaroenram W, Sriurairatana S, et al. Visual detection of white spot syndrome virus using DNA-functionalized gold nanoparticles as probes combined with loop-mediated isothermal amplification[J]. Molecular and Cellular Probes, 2013, 27(2):71-79.
[13] 白云,郑英帅,左奕,等. 胞内劳森菌的纳米PCR检测方法的建立[J]. 中国预防兽医学报,2016,12:972-975.
[14] 罗思思, 谢芝勋, 刘加波, 等. H7N9亚型AIV双重实时荧光定量RT-PCR检测方法的建立[J]. 动物医学进展, 2013, 34(12):1-5.
[15] Li H, Huang J, Lv J, et al. Nanoparticle PCR:Nanogold-assisted PCR with enhanced specificity[J]. Angewandte Chemie International Edition, 2005, 44(32):5100-5103.
[16] Li M, Lin Y C, Wu C C, et al. Enhancing the efficiency of a PCR using gold nanoparticles[J]. Nucleic Acids Research, 2005, 33(21):e184.
[17] 李家伟,郭利,杨勇,等. 牛病毒性腹泻病毒LAMP检测方法的建立与应用[J]. 中国畜牧兽医,2015,42(12):3111-3118.
[18] 郭利,王炜,张淑琴,等. 牛呼吸道疾病综合征相关病毒BVDV、BPIV3、IBRV和BRSV多重PCR检测方法的建立[J].特产研究,2014,1:12-15.
[19] 杨建乐. 牛副流感病毒3型间接ELISA检测方法的建立与初步应用[D].哈尔滨:东北农业大学,2016.
[20] 赵贵民,侯佩莉,贾春涛,等. 牛副流感病毒3型(BPIV3)P蛋白磷酸化位点与功能性结构域分析[J]. 中国兽医学报,2017,37(2):231-237.
[21] Boxus M, Letellier C, Kerkhofs P. Real Time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus[J]. Journal of Virological Methods, 2005, 125(2):125-130.
[22] Mayers J, Sawyer J. Development and evaluation of a multiplex enzyme-linked immunosorbent assay for the detection of antibodies to bovine respiratory diseases on the Meso Scale Discovery platform[J]. Journal of Veterinary Diagnostic Investigation, 2012, 24(4):725-729.
[23] 郑洋妹,陈信忠.环介导等温扩增技术检测动物病原研究进展[J].生物技术通报,2009,S1:108-112.

Establishment and Preliminary Application of Nano-PCR and LAMP Methods for Bovine Parainfluenza Type-3 Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FAN Lixia, YUAN Xuexia, LI Yuanyang, WU Yuanjuan, ZHAO Shancang, ZHANG Bingchun, WANG Wenbo. LAMP Detection and Drug Resistance Analysis to Staphylococcus aureus and Escherichia coli in Livestock and Poultry Manure [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2325-2335.
[2]	XIE Jing, YU Jifeng, ZHOU Long, CAO Ye, LIN Yi, LI Xingyu, XIAO Lu, YE Yonggang, PAN Meng, KANG Runmin. Establishment and Application of Visual LAMP Detection Method for Infectious Laryngotracheitis Virus [J]. , 2019, 46(2): 565-572.
[3]	XU Yunming, WEI Libin, ZHOU Yanyang, LI Yehui, SUN Zhiyuan, ZHONG Siyuan, CHEN Aining, GUO Lijuan, LIU Zengshan, REN Honglin. Establishment of Loop-mediated Isothermal Amplification (LAMP) Method for Klebsiella pneumoniae [J]. , 2018, 45(11): 3003-3010.
[4]	LIU Wenjun, HUANG Yunmao, YANG Youtian, XU Danning, CAO Nan, ZHOU Derong, TIAN Yunbo. Establishment of RT-LAMP Visual Detection Method for Goose Newcastle Disease Virus [J]. , 2018, 45(1): 22-31.
[5]	CHEN Xi-wen, WANG Qian, YIN Miao, LI Lian, LUO Wen-tao, YANG Feng, GUO Ai-wei, ZHOU Jie-long, WANG Xiong-qing. Establishment of RT-LAMP for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus [J]. , 2017, 44(6): 1630-1636.
[6]	LUO Ya-kun, LIANG Lin, WANG Jing, LIU Cun, LIU Qi, LIU Chang, LIN Wen-cheng, CUI Shang-jin. Establishment and Application of Loop-mediated Isothermal Amplification for Rapid Detection of Porcine Epidemic Diarrhea Virus [J]. , 2017, 44(2): 344-349.
[7]	WU Zhi, XIA Wen-long, CAI Shu-dong, GUO Chang-ming, YUAN Wei-feng, WANG Yong-juan. Establishment and Application of Visual RT-LAMP for Rapid Detection of Duck Tembusu Virus [J]. , 2017, 44(11): 3334-3339.
[8]	YANG Min, WANG Hui-dang, YU Lu, WU Chang-xin, Zhang Hui, CAO Xu-dong, CHEN Chuang-fu. Study on Optimization of the Conditions of Detecting Mycobacterium tuberculosis in Milk by Technique of Loop-mediated Isothermal Amplification [J]. , 2016, 43(7): 1688-1693.
[9]	WU Yu-xi, LI Pu-jun, WANG Juan, YANG Fa-long. Loop-mediated Isothermal Amplification Method and Its Application in Pathogen Detection [J]. , 2016, 43(2): 389-393.
[10]	WANG Cai-xia, FENG Chun-yan, WANG Qiao-li, YUAN Xiang-fen, DENG Jun-hua, LV Ji-zhou, WU Shao-qiang. Establishment of Duplex Loop-mediated Isothermal Amplification Method for Simultaneous Detection of Shellfish Infected with Perkinsus and Bonamia [J]. , 2015, 42(8): 1935-1942.
[11]	ZHANG Li, LI Kai, TIAN Guang-ling, LI De-feng, WANG Wei-song, HE Hou-jun. Development of Loop-mediated Isothermal Amplification Assay for Detection of Torque teno sus Virus Type 2 [J]. , 2015, 42(5): 1098-1103.
[12]	TANG Xiao-zhen, CHEN Lu-wei, LU Rong-bin, ZHENG Xin-tian, HUANG Cui-qin. Detection of Porcine Epidemic Diarrhea Virus by Calcein-based Visual Loop-mediated Isothermal Amplification (LAMP) Assay [J]. , 2015, 42(2): 331-336.
[13]	LI Jia-wei, GUO Li, YANG Yong, WANG Jian-ke, ZHANG Shu-qin, ZHANG Jia-li, CHENG Shi-peng. Establishment and Application of LAMP Detection Method of Bovine Viral Diarrhea Virus [J]. , 2015, 42(12): 3111-3118.
[14]	YANG Yi-ping, LIU Jian-hua, RAN Duo-liang, XU Quan-yuan, LI Na, Jiaerken. Establishment of Loop-mediated Isothermal Amplification Assay Detection Method of Equine Herpesviruses Type 1 [J]. , 2014, 41(9): 35-40.
[15]	QIU Suo-ping, LIN Zhi-xiong, YOU Yong-lai, MA Bao-hua, ZHAO Qiong. Development and Application of Loop-mediated Isothermal Amplification Assay for Salmonella Detection in Fecal Samples of Experimental Monkeys [J]. , 2014, 41(1): 51-56.