猪细环病毒2型环介导等温扩增检测方法的建立

doi:10.16431/j.cnki.1671-7236.2015.05.010

Abstract

Abstract: In order to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Torque teno sus virus type 2 (TTSuV2), two pairs of primers were designed according to the untranslated regions and the part of open reading frame 1 of TTSuV2.The LAMP system was optimized by adjusting the concentrations of some components and reaction conditions.The optimized amplification conditions of LAMP assay was at 64 ℃ for 90 min.The results showed the LAMP assay was specific for TTSuV2 detection, which could achieve a detection limit of 100 copies/μL viral nucleic acid, and no cross-reaction with TTSuV1, PCV2, CSFV, PRRSV and PBoV.In conclusion, this assay was a rapid, specific and sensitive detection technique which could provide a assistance for the rapid detection of TTSuV2.

Key words: Torque teno sus virus type 2 (TTSuV2); loop-mediated isothermal amplification (LAMP); detection

CLC Number:

S854.4

ZHANG Li, LI Kai, TIAN Guang-ling, LI De-feng, WANG Wei-song, HE Hou-jun. Development of Loop-mediated Isothermal Amplification Assay for Detection of Torque teno sus Virus Type 2[J]. , 2015, 42(5): 1098-1103.

[1]	CHEN Jimin, LI Huihuang, LAI Lianjie, YANG Yinhua, YANG Yue, LIN Ruiyi. Advance on Detection Methods and Vaccines of Duck Tembusu Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1329-1339.
[2]	HE Lin, HAN Diangang, LI Jing, DU Xuan, ZHANG Jiaxiang, XIN Jige. The Progress of the Detection Methods of Bovine Viral Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1593-1602.
[3]	ZHANG Qingxun, ZHONG Zhenyu, GUO Qingyun, HE Hongxuan, BAI Jiade. Recent Advances of Pathogens Detection Based on CRISPR-Cas System [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3190-3199.
[4]	WANG Qianqian, YU Yongle, LI Yuehua, DONG Yaqin, NI Bo, LIU Fuxiao. Advance in Detection Methods and Vaccines of Senecavirus A [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2336-2346.
[5]	GUO Luanying, LI Jialei, JIN Qinqin, MA Jun, LIU Quan. Research Progress on Bovine Hepacivirus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1994-2000.
[6]	LIANG Qian, BAO Taotao, XIAN Simei, YANG Qian, LI Pengfei, GU Qinglin, ZHENG Weihao, DU Peng, QING Chengxin. Establishment of PMA-PCR Method for Detection of Orf Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1524-1531.
[7]	LI Si, JIANG Wei, CHENG Tingting, LU Chunhui, XIN Sipei, CAO Jingzheng, WANG Quan, SUN Weidong. Establishment of an Indirect Competitive Chemiluminescence Enzyme-linked Immunoassay for Sarafloxacin [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1772-1783.
[8]	LI Xinping, CHEN Xiaojie, ZHAO Bing, LIU Qiangde, DING Zhanqiang, ZHANG Fei, TANG Huifen, CAO Jian, HOU Feng, LIU Yang, WANG Gang. Prokaryotic Expression of Clostridium chauvoei CctA Gene and Establishment of Its Indirect ELISA [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1876-1882.
[9]	HU Ruirui, LIU Li, GUO Tao, LI Yaxin, WANG Xiaokui, HU Shengwei. Establishment of a Visual Detection Technique for B2 Mutation of BMP15 Gene in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 810-817.
[10]	WU Shuaibin, CUI Zehua, LIAO Xiaoping, LIU Yahong, SUN Jian. Research Progress on the Detection Methods of Carbapenemase-producing Enterobacteriaceae [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1072-1083.
[11]	YANG Songxin, FENG Jianyuan, GUO Xuan, WEI Liuyuan, LIANG Yiying, YAN Hao, ZHANG Ziyi, CHEN Hailan. Preparation of Monoclonal Antibodies for Small Molecular Compounds and Their Applications in the Field of Veterinary Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1121-1131.
[12]	SHI Meiyi, YANG Falong. RPA and Its Application in Detection of Animal Pathogens [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 467-476.
[13]	YAN Hao, FENG Jianyuan, ZHANG Ziyi, YANG Songxin, XU Chunyu, WEI Meng, HUANG Qingping, CHEN Hailan. Progress in Preparation and Clinical Application of Nanobody [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 685-694.
[14]	YIN Yongkang, WANG Xiaoliang, CUI Yanli, LIANG Xiao. Research Progress on the Detection and Analysis Methods of Nicarbazine in Poultry Products and Feed [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4725-4734.
[15]	LIU Ang, CHENG Yiwen, AN Qi, ZHANG Zhenxing, LI Bin, CHEN Jie, CHEN Qiaoling, DU Li, MAN Churiga, WANG Fengyang, CHEN Si. Establishment of a Rapid Diagnostic Method of RPA for Pasteurella multocida from Goat [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3752-3760.

Development of Loop-mediated Isothermal Amplification Assay for Detection of Torque teno sus Virus Type 2

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments