过滤器辅助样品前处理法测定乳脂肪球膜蛋白质的研究

Abstract

Abstract: Bovine milk is consumed widely in the world. It provides multiple nutrients to human, including protein, fat, carbohydrate, vitamins and minerals. Protein is a major component of bovine milk. It contains caseins, whey protein and milk fat globule membrane protein (MFGM). Milk fat exists as milk fat globules in bovine milk and covered by a three-layer membrane which consists of lipids and protein. Recently, the identification of milk proteins gained interests. As the development of proteomic techniques, the number of identified milk protein is increasing. However, no fast and efficient method has been arisen to identify the MFGM proteins because of their hydrophobicity. Filter-aided sample preparation and NanoLC-MS/MS were combined and applied to study MFGM protein in present study. By using these methods, it is now possible to study MFGM in a fast and efficient way.

Key words: milk proteins; proteomics; filter-aided sample preparation; mass spectrometry

CLC Number:

Q516

LU Jing, LIU Lu, ZHANG Shu-wen, LV Jia-ping. Use of Filter Aided Sample Preparation to Study Milk Fat Globule Membrane Proteins[J]. , 2013, 40(S1): 93-100.

References

1 Affolter M, Grass L, Vanrobaeys F, et al. Qualitative and quantitative profiling of the bovine milk fat globule membrane proteome [J]. Journal of Proteomics, 2009, 73(6):1079~1088.
2 Bianchi L, Puglia M, Landi C, et al. Solubilization methods and reference 2-DE map of cow milk fat globules [J]. Journal of Proteomics, 2009, 72(5):853~864.
3 Casado B, Affolter M, Kussmann M. OMICS-rooted studies of milk proteins, oligosaccharides and lipids [J]. Journal of Proteomics, 2009, 73(2):196~208.
4 Dewettinck K, Rombaut R, Thienpont N, et al.Nutritional and technological aspects of milk fat globule membrane material [J]. International Dairy Journal, 2008,18(5):436~457.
5 Fong B Y, Norris C S, MacGibbon A K H. Protein and lipid composition of bovine milk-fat-globule membrane [J]. International Dairy Journal, 2007,17(4):275~288.
6 Gimeno R E,Cao J. Thematic review series: Glycerolipids. Mammalian glycerol-3-phosphate acyltransferases: New genes for an old activity [J]. Journal of Lipid Research, 2008, 49(10):2079~2088.
7 Gygi S P, Corthals G L, Zhang Y, et al. Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology [J]. Proceedings of the National Academy of Sciences, 2000, 97(17):9390~9395.
8 Mather I H,Keenan T W. Origin and secretion of milk lipids [J]. Journal of Mammary Gland Biology and Neoplasia, 1998, 3(3):259~273.
9 McManaman J L,Neville M C. Mammary physiology and milk secretion [J]. Advanced Drug Delivery Reviews, 2003, 55(5):629~641.
10 Peng J, Elias J E, Thoreen C C, et al. Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: The yeast proteome [J]. Journal of Proteome Research, 2003, 2(1):43~50.
11 Reinhardt T A, Lippolis J D. Bovine milk fat globule membrane proteome [J]. Journal of Dairy Research, 2006, 73(4):406~416.
12 Smolenski G, Haines S, Kwan J D,et al. Characterisation of host defence proteins in milk using a proteomic approach [J]. Journal of Proteome Research, 2007, 6(1):207~215.
13 Stenmark H,Olkkonen V M. The Rab GTPase family [J]. Genome Biol, 2001, 2(5):S3007.
14 Vanderghem C, Blecker C, Danthine S, et al. Proteome analysis of the bovine milk fat globule: Enhancement of membrane purification [J]. International Dairy Journal, 2008, 18(9):885~893.
15 Vuong G L, Weiss S M, Kammer W, et al. Improved sensitivity proteomics by postharvest alkylation and radioactive labeling of proteins [J]. Electrophoresis, 2000, 21(13):2594~2605.
16 Wi As' niewski J R, Zougman A, Nagaraj N, et al. Universal sample preparation method for proteome analysis [J]. Nature Methods, 2009, 6(5):359~362.

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Use of Filter Aided Sample Preparation to Study Milk Fat Globule Membrane Proteins

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