PCR技术在乳及乳制品致病菌检测中的应用

Abstract

Abstract: PCR technology is an in vitro DNA amplification technique born in 1985,with specificity,high sensitivity,simple, and rapid characteristics and has been widely used in various fields of biological sciences.The author describes the basic principles of PCR technology,its application in several pathogenic bacteria such as Staphylococcus aureus,Salmonellla spp,Shigella,Listeria monocytogenes and other harmful microorganisms detection in milk and dairy products.

Key words: PCR; pathogenic bacteria; milk and dairy products; detection

CLC Number:

TS252.7

LIU Hong-fang, HE Xian-shu, DUAN Yu-jun, YANG Yu, HAN Guo. Application of PCR Technique for Detection of Pathogenic Bacteria in Milk and Dairy Products[J]. , 2013, 40(S1): 74-77.

References

1 王晓闻, 杨永莉.免疫磁珠捕获-PCR检测牛乳中沙门氏菌的研究[J].山西农业大学学报(自然科学版), 2009, 29(4):289~293.
2 吕艳, 王华, 王君玮, 等.牛奶中金黄色葡萄球菌PCR检测方法的建立与应用[J].现代生物医学进展, 2009, 9(5):931～933.
3 孙焕冬, 李君文, 孙保国, 等.半套式聚合酶链反应检测牛奶中部分肠道致病菌[J].职业与健康, 2002, 18(3):43～44.
4 张巧艳, 陈亭亭, 陈笑芸, 等.基于SYBR Green Ⅰ荧光定量PCR建立生乳及乳制品沙门氏菌快速检测技术[J].浙江农业学报, 2012, 24(5):914~921.
5 张曼, 王晶钰.牛乳中产气荚膜梭菌α毒素和金黄色葡萄球菌肠毒素A的双重PCR检测法[J].西北农业学报, 2010, 19(9):29～33.
6 杨军, 张驰, 刘新梅.多重PCR法对乳制品中3种致病菌的同时快速检测[J].中国乳品工业, 2010, 38(4):50~53.
7 杨毓环, 马群飞, 王进喜.阪崎肠杆菌引起利乐包纯牛奶腐败变质及其卫生学意义[J].中国卫生检验杂志, 2002, 12(6):699~700.
8 苏世彦, 庄平, 陈忘名.食品微生物检验手册[M].北京:中国轻工业出版社, 1998.
9 陈伟, 杨迎伍, 邓伟, 等.三种致病菌多重PCR检测体系的建立及应用[J].食品与发酵工业, 2008, 34(9):132~136.
10 陈庆森, 冯永强, 黄宝华, 等.食品中致病菌的快速检测技术研究现状与进展[J].食品科学, 2004, 24(11):148~152.
11 胡建华, 李洁莉, 马兆飞, 等.牛奶样品中志贺氏菌的快速PCR检测技术研究[J].食品科学, 2007, 28(08):433~437.
12 赵宁, 吕琦, 姚丽燕, 等.原料乳中蜡样芽孢杆菌的快速检验[J].中国乳品工业, 2009, 37(9):43~45.
13 钟伟军, 赵明秋, 张彩虹, 等.食品中沙门氏菌PCR快速检测方法的建立[J].中国人兽共患病学报, 2007, 23(12):1216~1221.
14 高旗利, 张霞, 罗茂凰, 等.奶粉中阪崎肠杆菌PCR检测方法研究[J].检验检疫科学, 2005, 15(4):4~8.
15 唐吉思, 布日额, 吴金花, 等.荧光定量PCR检测牛乳中金黄色葡萄球菌肠毒素A基因方法的建立[J].中国预防兽医学报, 2012, 34(1):56~59.
16 梁会营, 侯殿东, 胡英, 等.婴幼儿奶粉中病原菌多重PCR检测研究[J].中国热带医学, 2008, 8(10):1685~1686, 1691.
17 覃艳华, 谢和, 胡萍, 等.原料奶中金黄色葡萄球菌分离鉴定及产毒菌株的快速检测[J].贵州农业科学, 2011, 39(5):140～143.
18 程媛媛, 张彦明, 向华, 等.牛奶中布鲁氏菌和单增李斯特菌双重PCR检测方法的建立[J].西北农林科技大学学报, 2011, 39(3):54~60.
19 戴岚, 宋白薇.含乳冷饮中阪崎肠杆菌和其它肠杆菌的污染状况[J].中国卫生检验杂志, 2009, 19(2):337~338.
20 Hege K N, Knut R, Kristine N, et al.Application of 5'-nuclease PCR for quantitative detection of listeria monocytogenes in pure cultures, water, skim milk, and unpasteurized whole milk[J].Applied and Enviromental Microbiology,2000, 66(10):4266~4271.
21 Malorny B, Wagener M.Detection of Enterobacter sakazakii strains by real-time PCR[J].Journal of Food Protection, 2005, 68(8):1623~1627.
22 Murphy B P, Buckley J F, O'Connor E M, et a1.Comparison of Salmonella species recovered from Irish liquid milk production holdings with temporal clinical veterinary isolates[J].International Journal of Hygiene and Environmental Health, 2008, 211:283~291.
23 Nero L A, de Mattos M R, de Aguiar Ferreira Barros M, et a1.Interference of raw milk autochthonous microbiota on the per-formance of conventional methodologies for Listeria monocyto-genes and Salmonella spp.detection[J].Microbiological Re- search, 2009, 164:529~535.

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Application of PCR Technique for Detection of Pathogenic Bacteria in Milk and Dairy Products

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