Abstract: The antigen,antibody microarray for detection of drug residues in raw milk were currently very few relevant reports,were now commercially available chip portfolio that there were all kinds of antibiotics, insect repellent combination chip,growth-promoting agent and combination. In this experiment,which could simultaneously quantitative determination of 15 kinds of combinations of sulfonamides chip experimental study, drug residues in raw milk in the chip accuracy,precision and so on would be studied. The results showed that the linear range was 0 to 20 ng/mL,two horizontal spiked (2 times the detection limit and 5 or 10 times the detection limit dosage), their recoveries mostly were in the 80% to 120%, which the latter was better than the former, through previous levels spiked 10 times measurement results,the coefficient of variation mostly was in the 5% to 15%. Through the determination of all the negative samples below the respective detection limits,quality control samples were measured to the reference value of 80% to 110%. Prove sulfa biochip could meet the performance indicators of drug residues raw milk semi-quantitative rapid screening,screening for bulk samples to provide a reliable technical guarantee.

Key words: protein biochip; raw milk; sulfonamides