鸭源多杀性巴氏杆菌对磺胺类药物的耐药性检测及耐药基因分析

doi:10.16431/j.cnki.1671-7236.2019.01.026

Abstract

Abstract:

In order to understand the antimicrobial sensitibity to sulfonamides and distribution of sulfonamides resistance genes of Pasteurella multocida strains isolated from ducks in Fujian,Guangdong,Jiangxi,etc,89 Pasteurella multocida strains were tested for antimicrobial susceptibility to trimethoprim-sulfamethoxazole by agar dilution method, PCR method and multi-locus sequence typing (MLST) were used for detection of sulfonamides resistance genes and to analyze the genetic relationship among the sulfonamides-resistant strains.The results showed that 33 strains (37.1%) were resistant to trimethoprim-sulfamethoxazole.Among the 33 sulfonamides-resistant strains,the detection rates of sul1,sul2 and sul3 genes were 100%(33/33),97.0%(32/33) and 21.2%(7/33).The simultaneous detection rate of sul1 and sul2 genes were 97.0%(32/33).The simultaneous detection rate of sul1,sul2 and sul3 genes were 21.2%(7/33).All of the 33 sulfonamides-resistant strains belonged to the ST129 sequence type.The results indicated that there was sulfonamides resistance to Pasteurella multocida isolated from ducks,and all the sulfonamides-resistant strains,which belonged to the same sequence type ST129,carried sul1 resistant gene,most of sulfonamides-resistant strains carried sul1 and sul2 resistant genes,suggesting that it should be rationally used sulfonamides to prevent and cure the infection of Pasteurella multocida isolated from ducks in some areas of Fujian,Guangdong and Jiangxi,etc.

Key words: Pasteurella multocida; sulfonamides; MLST; sul1 gene; sul2 gene; sul3 gene

CLC Number:

S852.61⁺2

CHEN Hongmei, CHENG Longfei, DENG Hui, LIU Rongchang, FU Guanghua, SHI Shaohua, FU Qiuling, WAN Chunhe, ZHENG Qingli, HUANG Yu, HUANG Xiaohong. Detection of Sulfonamides Sensitivity and Analysis of Resistant Genes of Pasteurella multocida Isolated from Ducks[J]. , 2019, 46(1): 223-230.

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Detection of Sulfonamides Sensitivity and Analysis of Resistant Genes of Pasteurella multocida Isolated from Ducks

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