China Animal Husbandry & Veterinary Medicine ›› 2013, Vol. 40 ›› Issue (S1): 37-38.

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Determination of Sodium Hydrogen Diacetate in Raw Milk using High Performance Liquid Chromatography

ZHANG Yang-dong1,2,3, ZHENG Nan1,2,3, LI Song-li1,2,3, ZHENG Shan-shan1,2,3, XU Xiao-min1,2, WANG Jia-qi1,2,3   

  1. 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Ministry of Agriculture-Milk Risk Assessment Laboratory, Beijing 100193, China;
    2. Ministry of Agriculture-Milk and Dairy Product Inspection Center, Beijing 100193, China;
    3. State Key Laboratory of Animal Nutrition, Beijing 100193, China
  • Received:2013-08-20 Online:2013-09-30 Published:2013-11-29

Abstract: A high performance liquid chromatography (HPLC) method was developed for determination of sodium hydrogen diacetate in raw milk. Protein was denatured with phosphoric acid then precipitated, and transfer sodium hydrogen diacetate into acetic acid in raw milk, constant volume, centrifuged and the supernatant sample over the membrane. The target compound was analyzed by HPLC with ammonium dihydrogen phosphate as mobile phase, the target was identified and determined by external standard method. In the concentration range of 0.05 to 0.50 mg/mL, R2 of calibration curve for the object was above 0.99. The recovery of target compound ranged from 90% to 110% with relative standard deviations of 1.0% to 4.1%. The detected limit was 0.05 g/kg,

Key words: HPLC; raw milk; sodium hydrogen diacetate

CLC Number: