高效液相色谱紫外法同时检测鸡蛋中5种青霉素类药物残留的研究

doi:10.16431/j.cnki.1671-7236.2017.08.037

Abstract

Abstract:

This study was aimed to develop a method for simultaneous determination of five kinds of penicillins in eggs by high performance liquid chromatography-ultraviolet detection (HPLC-UVD). The eggs samples were extracted by acetonitrile,degreased by n-hexane,concentrated by rotary evaporation,derivatization,and then was separated by HPLC. The results showed that the calibration curves of five kinds of penicillins were linear correlated in the concentration ranges of 10 to 1 000 μg/kg with the correlation coefficients >0.999,which the limits of detection and quantification were 1.5 μg/kg (S/N=3) and 5.0 μg/kg (S/N=10),respectively. The average recoveries were 62.04% to 93.13%,the relative standard deviations were 3.91% to 13.69%.And the intra-day and inter-day RSDs were 3.98% to 14.71% and 4.26% to 12.71%,respectively. The developed method was suitable for detecting five kinds of penicillins residues in eggs with high repeatability and sensitivity.

Key words: penicillin; high performance liquid chromatography-ultraviolet detection (HPLC-UVD); drug residues; eggs

CLC Number:

S859.7

LI Lei-ting, LIU Jun-hua, LIU Bao-tao, FU Hong-lei, ZOU Ming. Residue Analysis of Five Kinds of Penicillins in Eggs by High Performance Liquid Chromatography-Ultraviolet Detection[J]. , 2017, 44(8): 2483-2488.

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Residue Analysis of Five Kinds of Penicillins in Eggs by High Performance Liquid Chromatography-Ultraviolet Detection

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