基于实时荧光定量反转录PCR技术的动物miRNA表达分析策略

Abstract

Abstract: About 60% of human protein-coding genes are regulated by miRNA. These genes produce different proteins, which participant in many biological processes. The functions of these proteins are involved in cell cycle,development,differentiation and metabolism. Nowadays, researchers can detect the expressions of miRNA by various approaches. However, the RT-PCR-based amplifying techniques are the most flexible methods, which are applied in extensive fields. The RT-PCR-based amplifying techniques mainly include miR-Q RT-PCR for miRNA quantification, end-point stem-loop Real-time RT-PCR for miRNA quantification, Poly(A)-tailed universal reverse transcription, multiplexing RT-PCR for high-throughput miRNA profiling and miRNA amplification profiling (mRAP). Here we introduce these important Real-time PCR methods for detecting miRNA, respectively. And the theory, strategy, application and limitation of each method are summarized synoptically.

Key words: animal miRNA; Real-time PCR; miRNA quantitative RT-PCR; stem-loop primer; miRNA amplification profile

CLC Number:

SONG Jia-zhe, SUN Fu-bo, ZHUANG Kai-ge, XUE Kai. Real-time Quantitative RT-PCR Analysis of Animal miRNA Expression[J]. , 2013, 40(9): 109-113.

References

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Real-time Quantitative RT-PCR Analysis of Animal miRNA Expression

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