牦牛PLIN2基因的克隆及其在卵巢不同发情阶段的表达

doi:10.16431/j.cnki.1671-7236.2017.03.023

Abstract

Abstract:

The purpose of this study was to research the sequence characteristics and the expression of PLIN2 gene, and to analyze the relationship between the expression and the ovaries in different estrus periods. According to the gene sequence of bovine in GenBank, the primer was designed. The PLIN2 gene of yak was cloned by RT-PCR, and the expression quantity of the PLIN2 gene in different tissues of yak was detected by the semiquantitative RT-PCR. Meanwhile,the differential expression of the PLIN2 gene in different ovarian estrus periods of yak was detected by quantitative Real-time PCR. The results showed that the length of the PLIN2 gene was 1 353 bp in CDS, encoding 450 amino acids. The PLIN2 protein was predicted to be non-seretory and lacking cross-membrane domain. It was 99.4% homology identity to that of corresponding cDNA from bovine, which showed that the PLIN2 gene was quite conservative in the process of evolution. The expession profile of PLIN2 was wide in yak tissues, but there were some differences in different tissues. The expression quality was higher in kidney, breast and ovary, while it was almost nothing in lung. The result of quantitative Real-time PCR indicated that yak PLIN2 gene was expressed in different ovarian estrus periods, and the highest expression during the luteal phase, which indicated the PLIN2 gene played an essential role in yak estrous cycles.

Key words: yak; PLIN2 gene; clone; expression in tissue; quantitative Real-time PCR

CLC Number:

Q785

CAI Wen-yi, CHEN Tong, SHI Xian, CHEN Wei-ming, HU Jia-jia, XIONG Xian-rong, LAN Dao-liang, LI Jian. Cloning of PLIN2 Gene of Yak and Its Expression in Ovary during Different Estrus Periods[J]. , 2017, 44(3): 782-789.

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Cloning of PLIN2 Gene of Yak and Its Expression in Ovary during Different Estrus Periods

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