弓形虫不同发育时期棒状体颈部蛋白5基因的表达研究

doi:10.16431/j.cnki.1671-7236.2017.02.007

Abstract

Abstract:

To investigate the relationship between the expression level of Toxoplasma gondii rhoptry neck protein 5 (TgRON5) gene in different developmental stages and the virulence of Toxoplasma gondii, the objective of this study was to examine the different expression of TgRON5 gene in different developmental stages of type Ⅱ Toxoplasma gondii PRU strain. Specific primers were designed according to the sequence of TgRON5 gene, and ACT1 gene of Toxoplasma gondii was used as a reference gene. Following the establishment of standard curve for the target and reference genes, in order to confirm the consistency of their amplification efficiency, Real-time PCR method was applied to determine and compare the expression level of TgRON5 gene in development stage which including tachyzoite, bradyzoite, non-sporulated oocyst and sporulated oocyst. The results demonstrated that TgRON5 was expressed in all developmental stages but the sporulated oocysts had the highest expression, followed by the non-sporulated oocysts, the third was tachyzoite, and the lowest was bradyzoite. It suggests that the synthesis and secretion of TgRON5 protein was closely associated with the invasiveness of the parasite. All these findings had important implications for elucidating the functions of TgRON5 involved in the invasion of Toxoplasma gondii.

Key words: Toxoplasma gondii; rhoptry neck protein 5; Real-time PCR; expression level

CLC Number:

Q786

HE Shuai, ZHOU Chun-xue, ZHU Xing-quan, ZHOU Dong-hui, XU Qian-ming. Study on Expression of Toxoplasma gondii Rhoptry Neck Protein 5 Gene in Different Developmental Stages[J]. , 2017, 44(2): 350-356.

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Study on Expression of Toxoplasma gondii Rhoptry Neck Protein 5 Gene in Different Developmental Stages

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