豆状带绦虫线粒体rrnL和nad5基因的克隆及种系发育研究

Abstract

Abstract: The objectives of the present study were to examine sequence variation in the mitochondrial large subunit ribosomal rRNA (rrnL) and NADH dehydrogenase subunit 5 (nad5) genes among Taenia pisiformis isolates from Henan province and study its phylogenetic relationships with other cestodes using rrnL and nad5 sequences. The partial rrnL (prrnL) and nad5 (pnad5) genes sequences were amplified from each T.pisiformis sample by polymerase chain reaction (PCR), and prrnL and pnad5 sequences were aligned using the ClustalX 1.81. Maximum parsimony (MP) tree was constructed using the software PAUP 4.0 Beta 10. The lengths of prrnL and pnad5 sequences were 847 and 602 bp, respectively. Sequence similarity in rrnL among the examined T.pisiformis samples were 99.65%, and 99.5% for nad5, whereas inter-specific similarity among taeniid cestodes were significantly lower than 83.4% for rrnL, and lower than 85.7% for nad5. Phylogenetic analysis showed that all the T.pisiformis isolates clustered in the same clade. It was concluded that prrnL and pnad5 sequences could be used as a genetic marker for the identification and differentiation of taeniid cestodes.

Key words: Taenia pisiformis; mitochondrial DNA; rrnL gene; nad5 gene; phylogenetic relationship

CLC Number:

FAN Yi-fan, LIU Guo-hua, YANG Yan-chuan, HAN Jin-huan, ZHU Xing-quan, JIANG Wen-can, SUN Xiao-lin. Cloning and Phylogenetic Analysis of Mitochondrial rrnL and nad5 Genes of Taenia pisiformis Isolates[J]. , 2013, 40(6): 45-49.

References

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Cloning and Phylogenetic Analysis of Mitochondrial rrnL and nad5 Genes of Taenia pisiformis Isolates

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