冷藏蓝舌病样品的Real-time PCR检测和病毒颗粒稳定性研究

Abstract

Abstract: In order to understand the stability of blue-tongue virus (BTV)storage in cold room for long time, 18 tissue samples which were kept in 4℃ for 10 to 15 years were test by Real-time PCR, and 10 blood samples which were kept in 4℃ for 15 years were subjected to virus isolation process, then was proved by monoclonal antibody staining,research results showed all old samples were positive in Real-time PCR, further more, 5 live virus were isolated from 10 old blood samples. The results suggested the stability of BTV was higher than we thought before.

Key words: blue-tongue virus; storage in cold room; stability

CLC Number:

MIAO Hai-sheng, LI Le, ZHU Jian-bo, XIAO Lei, LI Hua-chun. Study on the Stability of Blue-tongue Virus after Long Time Storage in Cold Room[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(6): 41-44.

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Study on the Stability of Blue-tongue Virus after Long Time Storage in Cold Room

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