猪繁殖与呼吸综合征病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立

Abstract

Abstract: A pair of specific primers conserved in the the Nsp7 genes of American porcine reproductive and respiratory syndrome virus (PRRSV) were synthesized. Optimization of reaction conditions, the detection of SYBR Green Ⅰ Real-time quantitative PCR method was compared and analyzed at the same time with the commercialization of fluorescent quantitative PCR kit. The results showed that the specificity of the method, high sensitivity, a good distinction between the American swine reproductive and respiratory syndrome virus and other viruses. 14 clinical samples were detected using this method, the positive rate was 85.7%, coincidence was 100% with commercial kits.

Key words: porcine reproductive and respiratory syndrome virus; Real-time quantitative PCR; Nsp7

CLC Number:

S854.4⁺3

HUANG Zhen, WEI Chun-ya, YAN Ming-fei, TIAN Yue-ru, ZHANG Min-ze, XIE Jie-xiong, SUN Long, ZHANG Gui-hong. Establishment of SYBR Green Ⅰ Fluorescent Quantitative PCR Method for Detection of Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2013, 40(4): 89-92.

[1]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[2]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[3]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[4]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[5]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[6]	WANG Zhaoli, YANG Siyu, WU Yanmei, SONG Xiaoxiao, MU Xiang, ZHANG Tao. Effects of PRRSV-LPS Treatment on Neutrophil Adhesion to Porcine Pulmonary Microvascular Endothelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 471-479.
[7]	SUN Lu, ZHANG Jing, YIN Miao, CHENG Yaodie, DAI Kun, WU Qian, ZHAO Hong, CHEN Xiwen. Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 746-754.
[8]	CHU Pinpin, JIANG Zhiyong, LI Chunling, GOU Hongchao, LI Yan, CAI Rujian. Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4356-4363.
[9]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[10]	MIAO Manning, GUO Yiwen, HU Debao, ZENG Yuhan, LI Xin, ZHANG Linlin, DING Xiangbin, GUO Hong. Transcriptome Sequencing and Bioinformatics Analysis of MSTN Gene-edited Bovine Muscles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2271-2281.
[11]	LI Heng, ZI Xiangdong. Cloning and Expression in Cumulus-oocyte Complexes of Yak TEDDM1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1284-1293.
[12]	CHEN Lei, YUAN Feng, HE Sangang, MAYILA, LI Wenrong. Cloning,Sequencing and Quantitative Expression of FGF10 Gene in Sheep During Hair Follicle Development Period [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 425-432.
[13]	DAI Zhenglie, ZHU Jingjing, CHEN Zhenyu, ZHOU Xiaolong, WANG Han, LI Xiangchen, ZHAO Ayong, YANG Songbai. Cloning, Bioinformatics Analysis and Expression of Porcine SENP1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4382-4390.
[14]	JIANG Yixuan, WANG Yaling, XING Shanshan, SONG Guohua, XU Huifen, LI Ming. Cloning, Bioinformatics Analysis and Expression Profile Construction of MSTN Gene in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3501-3511.
[15]	LI Zhiqiang, TAO Chenyu, WEI Aolong, JIA Qing. Establish on Real-time Quantitative PCR Method for Purity Detection of Porcine Sex-controlled Sperm [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3682-3690.

Establishment of SYBR Green Ⅰ Fluorescent Quantitative PCR Method for Detection of Porcine Reproductive and Respiratory Syndrome Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments