Abstract: The experiment was conducted to investigate the effects of TFHL, TSG and TFHL+TSG supplement in daily ration for broilers on growth performance, utilization rate of nutrients, digestive enzyme activity and intestinal bacterial.128 male broilers (1 day old) were divided randomly into 4 groups, the control group (fed a basal corn-soybean meal diet), groupⅠ (fed a basal corn-soybean meal diet containing 300 mg/kg TFHL), groupⅡ (fed a basal corn-soybean meal diet containing 150 mg/kg TSG) and group Ⅲ(fed a basal corn-soybean meal diet containing 300 mg/kg TFHL and 150 mg/kg TSG). Each group had 4 replacements, each replacement had 8 broilers. The results showed that:compared with the control group, the ADG in group Ⅲ significantly increased (P<0.05) during the whole experimental periods (1 to 42 days),and the F/G reduced significantly (P<0.05). The digestive utilization ratios of CP, Ca and TP in group Ⅲ were significantly higher than that in control,Ⅰ and Ⅱ groups (P<0.05); the EE digestive utilization ratio was significantly lower than that in control, control,Ⅰ and Ⅱ groups (P<0.01). Compared with the control and Ⅱ groups ,the Trypsin activity in group Ⅲ increased significantly (P<0.01); compared with the control group, the lipase activity significantly lower (P<0.01); compared with the control and Ⅰ groups, the amylase activity was significantly increased (P<0.01). Compared with the control,Ⅰand Ⅱ groups, the coliform counts in ileum and cecum significantly reduced in group Ⅲ(P<0.05). Compared with the control group, the lactic acid bacteria number of ileum in group Ⅲ significantly increased(P<0.05); compared with the control,Ⅰ and Ⅱ groups, the lactic acid bacteria number of cecum in group Ⅲ significantly increased(P<0.05). Supplement of TFHL or TSG in diet played a role in improving production trait and utility ratio of digestion, enhancing activity of intestinal trypsin and amylase, reducing activity of lipase, stimulating growth of intestinal beneficial bacterium and inhibiting reproduction of pathogen for broiler. Taking all factors into consideration, it was better to add the two ingredients to diet at the same time.

Key words: TFHL; TSG; broiler; growth performance; digestive utilization ratios of nutrients; digestive enzyme activity; intestinal bacterial