牦牛乳脂合成关键酶基因全泌乳期的表达研究

Abstract

Abstract: The Maiwa yak, which were used as milk producer in the northwest plateau of Sichuan province, were selected as experimental animal. The Real-time quantitive (RT-PCR) was applied to detect the expression level of ACSL1, ACSS1, ACSS2 and FABP3 genes in the mammary gland of yak during the lactation cycle. The results showed that ACSL1, ACSS1, ACSS2 and FABP3 gene were consistently expressed during the whole lactation period. RT-PCR results showed that the expression level of all 4 genes consistently increased from time point -15 d, and the peak appeared at time point 30 d. The expression level of all 4 genes at 30 d significantly increased (P<0.05) compared with time point -15 d. Meanwhile, the peak of milk yield was at time point 120, 90 d later than the peak of mRNA expression levels, then the milk yield consistently decreased till 240 d. These results implied that the expression pattern of milk fat synthesis gene during the lactation cycle reflected the yak’s genetic adaption to living environment in Tibetan plateau.

Key words: Maiwa yak; milk fat synthetic key enzyme genes; mammary gland; Real-time PCR

CLC Number:

Q786

LI Yu-can, DENG Xiao-yu, JIANG Ming-feng. Study on Expression of Key Enzyme Genes Related to Milk Fat Synthesis in Mammary Gland of Yak[J]. , 2013, 40(4): 59-64.

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Study on Expression of Key Enzyme Genes Related to Milk Fat Synthesis in Mammary Gland of Yak

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