奶牛乳腺中4F2hc基因的表达模式及亮氨酸对其表达的调控研究

doi:10.16431/j.cnki.1671-7236.2020.08.005

Abstract

Abstract: To investigate the expression and regulation of 4F2hc in mammary glands of dairy cows,and clarify the process of amino acids transport across plasma membrane of mammary epithelial cells,the expression of 4F2hc inmammary tissues of lactation cows and dry cows were detected by Western blotting and Real-time quantitative PCR.The mammary epithelial cells of lactating cows in vitro were treated with leucine,the effect of leucine on the expression of 4F2hc in the mammary epithelial cells of lactating cows was also detected by Western blotting and Real-time quantitative PCR.The expression of 4F2hc and the synthesis of milk protein in the mammary epithelial cells of dairy cows were detected by Western blotting.The results showed that the mRNA and protein expression levels of 4F2hc were significantly or extremely significantly higher in mammary tissues of lactating cows than that in dry cows (P<0.05,P<0.01).Adding leucine to cultured bovine mammary epithelial cells could extremely significantly increase the expression of 4F2hc mRNA and protein (P<0.01).Leucine stimulation could activate the mTOR signaling pathway (P<0.05),while rapamycin treatment could significantly inhibit the phosphorylation of mTOR signaling molecules and the expression of 4F2hc induced by leucine (P<0.05,P<0.01),and then extremely significantly inhibit the synthesis of β-Casein(P<0.01).These results revealed that the expression of 4F2hc was positively correlated with lactation activity in mammary glands of dairy cows.Leucine could regulate 4F2hc expression via mTOR signaling pathway in mammary epithelial cells of dairy cows,thus affecting milk protein synthesis.

Key words: dairy cows; mammary gland; 4F2hc gene; lactation

CLC Number:

Q786

JIANG Minghui, HUANG He, DUAN Xiaoyu, JIANG Yi, LIU Can, HOU Xiaoming. Expression Pattern of 4F2hc Gene in Mammary Glands of Dairy Cows and Regulation of Its Expression by Leucine[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2378-2384.

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Expression Pattern of 4F2hc Gene in Mammary Glands of Dairy Cows and Regulation of Its Expression by Leucine

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