水牛泌乳期与非泌乳期乳腺组织差异miRNAs的表达模式鉴定及分析

doi:10.16431/j.cnki.1671-7236.2017.11.016

Abstract

Abstract:

In order to explore the differential expression miRNAs and their expression patterns in the control of buffalo in lactation and non-lactation, the miRNAs of 18 buffalo breast tissues differentially expressed in lactation and non-lactation were screened by Solexa high-throughput sequencing and bioinformatics analysis. Viable miRNAs primers for reverse transcription and Real-time quantitative PCR were used to validate the expression patterns of miRNAs. The Real-time quantitative PCR results were basically consistent with sequencing results. The expression levels of miR-103, miRNA-125a, miRNA-30a-5p and miRNA-148a in lactation were significantly higher than those in non-lactation (P<0.05). The expression level of miRNA-29a in non-lactation was higher than that in lactation (P<0.05). The expression levels of miRNA-141, miRNA-125b and miRNA-497 in lactation were higher than those in non-lactation,but the difference was not significant (P>0.05). The low-expression levels of miRNA-490 and miRNA-592 in lactation were significantly higher than those in non-lactation (P<0.05). There was no significant difference in the expression of Novel-123b between lactation and non-lactation (P>0.05). Novel-57 was extremely significantly in lactation (29.79 times) higher than non-lactation (P<0.01). This results provide available specific reverse transcription primers and Real-time quantitative PCR primers of 16 miRNAs for further study on buffalo,and 6 miRNAs such as miRNA-103, miRNA-125a, miRNA-30a-5p, miRNA-148a,miRNA-29a and Novel-57 deserve further research.

Key words: buffalo; lactation; non-lactation; mammary gland; miRNA; identification; Real-time quantitative PCR

CLC Number:

S814.8

CAI Xiao-yan, WANG Peng-cheng, DENG Kai, WANG Ping, FENG Wan-you, ZHANG Xiao-xi, REN Yan-ping, LIU Qing-you, SHI De-shun. Identification and Analysis of Differential Expression miRNAs in the Lactation and Non-lactation of Buffalo Mammary Gland[J]. , 2017, 44(11): 3220-3228.

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Identification and Analysis of Differential Expression miRNAs in the Lactation and Non-lactation of Buffalo Mammary Gland

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