›› 2013, Vol. 40 ›› Issue (4): 45-49.

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Study on Genetic Structure of Dahe Black Pig Based on DNA Sequence Analyses of Cytochrome b Gene

TANG Li-zhou1, SHI Xiao-dong1, DING Wei1, YU Long1, WANG Jun-jie1, ZHU Lei1, GUO Song-chang2   

  1. 1. Yunnan-Guizhou Plateau Institute of Biodiversity, College of Biologic Resource and Environment Science, Qujing Normal University, Qujing 655011, China;
    2. Key Laboratory of the Qinghai-Tibetan Plateau Ecosystem and Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
  • Received:2012-09-27 Online:2013-04-20 Published:2013-04-19

Abstract: For revealing the level of genetic divergence and genetic structure of Dahe Black pig, this research was implemented using molecular biological methods that sequenced and analyzed the Cyt b DNA sequences based on 46 samples from 5 geographic populations of Qujing city. The AMOVA results showed that most of genetic variation (62.63%) distributed within populations. This could indicate fully that the whole population genetic structure of this species was not evident because of significant gene flow among different populations. The results of neutrality tests further validated that the instable population structure of this whole group could be affected by the population expansion from the artificial selective breeding and introduction breeding. This instable population structure or lower genetic differentiation could be induced by the large-scale introduction and expanding breeding. The significant gene flow induced the genetic convergence in various geographical populations eventually, population expanding and population genetic diversity declined significantly. This study was expected to provide important scientific basis and data for the molecular genetic breeding of Dahe Black pig and for the manager’s effective measures to protect the genetic resources of Dahe Black pig.

Key words: Dahe Black pig; mitochondrion gene; cytochrome b; genetic diversity; genetic structure

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