Abstract: In order to explore the expression of prostaglandin E₂ and F_2α synthase (PGES and PGFS) mRNA in bovine oviductal epithelial cells by estrogen,investigation the function of physiology in bovine. We used bovine regenerating epithelial cells in vitro training system,different times(0,2,4,8,16,24 and 48 h)and different concentrations of estrogen (0,10^-12,10^-11,10^-10 and 10^-9 mol/L) were added, and measured PGES and PGFS mRNA expression by using Real-time PCR. The results showed that different concentrations of E₂ or different stimulation time in the same concentration of E₂ could increase the expression of prostaglandin E₂ synthase (PGES), but in addition of E₂ 4 h and the concentration was 10^-10 mol/L compared with the control was extremly significant different (P<0.01), while PGFS in addition of E₂ 24 h and the concentration was 10^-12 mol/L compared with the control was extremly significant different (P<0.01).Experimental results showed that estrogen could promote the expression of PGES and PGFS mRNA in oviduct epithelial cells of bovine, and regulate the mRNA expression of prostaglandin synthase PGES and PGFS.

Key words: estrogen; oviductal epithelial cells; PGES; PGFS; Real time RT-PCR