雌激素对奶牛输卵管组织COX-1和COX-2表达的影响

doi:10.16431/j.cnki.1671-7236.2016.03.033

Abstract

Abstract: The aim of this study was to clarify the effects of estrogen (E₂) on cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) gene expression in the cow oviduct tissue in vitro.Quantitative Real-time PCR and Western blotting were used to analyze the mRNA and protein expression of COX-1 and COX-2 at different time points after different concentrations of E₂ treatment.The results indicated that the relative expression of COX-1 and COX-2 mRNA reached the highest levels with 10^-11 mol/L E₂ treatment,the relative expression of COX-1 mRNA reached the highest level at 8 h after treatment;The relative expression of COX-2 mRNA reached the highest level at 2 h after treatment.The relative expression of COX-1 protein increased significantly at the range from 8 to 48 h after E₂ treatment.However,we did not detect COX-2 protein expression.

Key words: oviduct; cyclooxygenase-1; cyclooxygenase-2; estrogen (E₂)

CLC Number:

Q786

SHEN Yuan, HUANG Na, LIU Bo, MAO Wei, CAO Jing-shan. Effects of Estrogen on COX-1 and COX-2 Expression in Bovine Oviduct Smooth Muscle[J]. , 2016, 43(3): 791-797.

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Effects of Estrogen on COX-1 and COX-2 Expression in Bovine Oviduct Smooth Muscle

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