›› 2015, Vol. 42 ›› Issue (6): 1333-1339.doi: 10.16431/j.cnki.1671-7236.2015.06.002

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Effect of PGE2 Receptor Agonist Butaprost and Estrogen on Expression of TGFβ3 in Bovine Oviduct Epithelial Cell

WANG Mao-mao1,2, MAO Wei1,2, CAO Jin-shan1,2, DONG Zhi-heng1,2, LIU Bo1,2   

  1. 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Key Laboratory of Animal Disease Clinical Diagnosis, Ministry of Agriculture, Hohhot 010018, China
  • Received:2014-12-25 Online:2015-06-20 Published:2015-07-23

Abstract: This study was aimed to observe effect of the prostaglandin receptor agonists butaprost and estrogen on TGFβ3 mRNA expression and illustrate whether butaprost and estrogen had coordinated regulation on bovine oviduct epithelial cells (BOECs) transforming growth factor β3 (TGFβ3) or not.BOECs were isolated and cultured by mechanical and pancreatic enzyme digestion method;butaprost and estrogen acted on BOECs,respectively;mRNA expression of TGFβ3 was detected by Real-time PCR in BOECs.The results showed that after the action of estrogen,the expression of TGFβ3 at 16,24 and 48 h compared with 0 h were extremely significantly increased (P< 0.01),TGFβ3 mRNA expression reached minimal at 4 h (P< 0.01);The receptor agonist butaprost and estrogen had coordinated regulation on TGFβ3.Adding indomethacin could inhibit the expression of endogenous prostaglandin for TGFβ3.The results showed that butaprost and estrogen could regulate the expression of TGFβ3 mRNA in BOECs.

Key words: butaprost; estrogen; bovine oviduct epithelial cells (BOECs); transforming growth factor β3 (TGFβ3)

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