Abstract: The physicochemical properties and proliferative characteristics in BHK-21 cells of Japanese encephalitis virus(JEV) LS strain were studied in the test. The results indicated that JEV LS strain was sensitive for temperature (56 ℃), acid(below pH 5.0), diethyl ether and trypsin. The viral titer of LS strain remained at the same level undergoing a repeated freeze-thaw test. The TCID₅₀ and hemagglutination titer of LS strain kept at the high level (TCID₅₀=10^7.75/mL,2⁸) after 20 serial passages in BHK-21 cells. According to the proliferation dynamic curve of JEV LS strain in BHK-21 cells, the optimum time to harvest JEV was 28 to 40 h after virus innoculation. This research laid a foundation for the further research on biological characteristics of JEV LS strain.

Key words: Japanese encephalitis virus; physicochemical properties; proliferation rule